Effects of antenatal endotoxin and glucocorticoids on the lungs of preterm lambs

Jobe, Alan H., Newnham, John P., Willet, Karen E., Sly, Peter, Ervin, M.Gore, Bachurski, Cindy, Possmayer, Fred, Hallman, Mikko and Ikegami, Machiko (2000) Effects of antenatal endotoxin and glucocorticoids on the lungs of preterm lambs. American Journal of Obstetrics and Gynecology, 182 2: 401-408. doi:10.1016/S0002-9378(00)70231-6

Author Jobe, Alan H.
Newnham, John P.
Willet, Karen E.
Sly, Peter
Ervin, M.Gore
Bachurski, Cindy
Possmayer, Fred
Hallman, Mikko
Ikegami, Machiko
Title Effects of antenatal endotoxin and glucocorticoids on the lungs of preterm lambs
Journal name American Journal of Obstetrics and Gynecology   Check publisher's open access policy
ISSN 0002-9378
Publication date 2000-02
Sub-type Article (original research)
DOI 10.1016/S0002-9378(00)70231-6
Volume 182
Issue 2
Start page 401
End page 408
Total pages 8
Place of publication Philadelphia, PA, United States
Publisher Mosby
Language eng
Formatted abstract
We hypothesized that the proinflammatory response to intra- amniotic endotoxin would induce lung maturation in preterm lambs.


Ewes were randomly assigned to receive 20 mg Escherichia coli endotoxin by intra-amniotic injection, maternal betamethasone (0.5 mg/kg), or sodium chloride solution. Preterm lambs were delivered at 125 days' gestation and underwent ventilation to assess lung function. Lung gas volume, surfactant concentrations, and inflammation were subsequently evaluated, with data analyzed by analysis of variance.

Fetal endotoxin exposure 6 days before delivery increased compliance by 59%, increased lung gas volume 2.3-fold, increased concentrations of surfactant lipids, increased surfactant A and B protein levels, and increased messenger ribonucleic acid expressions for surfactant proteins (all P < .01, vs control group). Betamethasone exposure resulted in less consistent effects. White blood cell counts were increased in fetal membranes and lungs after endotoxin exposure, but there was no severe inflammation.

A single fetal exposure to endotoxin resulted in large improvements in postnatal lung function and increases in surfactant concentrations after preterm delivery. These effects were qualitatively larger than those achieved with betamethasone.
Keyword Compliance
Lung maturation
Respiratory distress syndrome
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Available online 4 November 2005.

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 108 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Wed, 17 Nov 2010, 11:33:49 EST