Deficiency of SP-B reveals protective role of SP-C during oxygen lung injury

Ikegami, M., Weaver, T. E., Conkright, J. J., Sly, P. D., Ross, G. F., Whitsett, J. A. and Glasser, S. W. (2002) Deficiency of SP-B reveals protective role of SP-C during oxygen lung injury. Journal of Applied Physiology, 92 2: 519-526.

Author Ikegami, M.
Weaver, T. E.
Conkright, J. J.
Sly, P. D.
Ross, G. F.
Whitsett, J. A.
Glasser, S. W.
Title Deficiency of SP-B reveals protective role of SP-C during oxygen lung injury
Journal name Journal of Applied Physiology   Check publisher's open access policy
ISSN 8750-7587
Publication date 2002-02
Sub-type Article (original research)
Volume 92
Issue 2
Start page 519
End page 526
Total pages 8
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Language eng
Formatted abstract
Although the surface properties of surfactant protein (SP)-B and SP-C are similar, the contributions that either protein may make to lung function have not been identified in vivo. Mutations in SP-B cause lethal respiratory failure at birth; however, SP-B null mice are deficient in both SP-B and SP-C. To identify potential contributions of SP-C to lung function in vivo, the following transgenic mice were generated and exposed to 95% O2 for 3 days: (SP-B+/+,SP-C+/+), (SP-B+/+, SP-C-/-), (SP-B+/-,SP-C+/+), (SP-B+/-,SP-C+/-), and (SP-B+/-,SP-C-/-). Hyperoxia altered pressure-volume curves in mice that were heterozygous for SP-B, and these values were further decreased in (SP-B+/-,SP-C-/-) mice. Likewise, alveolar interleukin (IL)-6 and IL-1β were maximally increased by O2 exposure of (SP-B+/-,SP-C-/-) mice compared with the other genotypes. Lung hysteresivity was lower in the (SP-B+/--,SP-C-/-) mice. Surfactant isolated from (SP-B+/+,SP-C-/-) and (SP-B+/--,SP-C-/-) mice failed to stabilize the surface tension of microbubbles, showing that SP-C plays a role in stabilization or recruitment of phospholipid films at low bubble radius. Genetically decreased levels of SP-B combined with superimposed O2-induced injury reveals the distinct contribution of SP-C to pulmonary function in vivo.
Keyword Lung injury
Transgenic mice
Saturated phosphatidylcholine
Surfactant protein-B and -C
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
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Created: Wed, 17 Nov 2010, 11:33:19 EST