Methacholine responsiveness in mice from 2 to 8 wk of age

Bozanich, Elizabeth M., Janosi, Tibor Z., Collins, Rachel A., Thamrin, Cindy, Turner, Debra J., Hantos, Zoltan and Sly, Peter D. (2007) Methacholine responsiveness in mice from 2 to 8 wk of age. Journal of Applied Physiology, 103 2: 542-546. doi:10.1152/japplphysiol.01253.2006


Author Bozanich, Elizabeth M.
Janosi, Tibor Z.
Collins, Rachel A.
Thamrin, Cindy
Turner, Debra J.
Hantos, Zoltan
Sly, Peter D.
Title Methacholine responsiveness in mice from 2 to 8 wk of age
Journal name Journal of Applied Physiology   Check publisher's open access policy
ISSN 8750-7587
1522-1601
Publication date 2007-08
Sub-type Article (original research)
DOI 10.1152/japplphysiol.01253.2006
Volume 103
Issue 2
Start page 542
End page 546
Total pages 5
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Language eng
Abstract Many chronic human lung diseases have their origin in early childhood, yet most murine models used to study them utilize adult mice. An important component of the asthma phenotype is exaggerated airway responses, frequently modelled by methacholine (MCh) challenge. The present study was undertaken to characterize MCh responses in mice from 2 to 8 wk of age measuring absolute lung volume and volume-corrected respiratory mechanics as outcome variables. Female BALB/c mice aged 2, 3, 4, 6, and 8 wk were studied during cumulative intravenous MCh challenge. Following each MCh dose, absolute lung volume was measured plethysmographically at functional residual volume and during a slow inflation to 20-hPa transrespiratory pressure. Respiratory system impedance was measured continuously during the inflation maneuver and partitioned into airway and constant-phase parenchymal components by model fitting. Volume-corrected (specific) estimates of respiratory mechanics were calculated. Intravenous MCh challenge induced a predominantly airway response with no evidence of airway closure in any age group. No changes in functional residual volume were seen in mice of any age during the MCh challenge. The specific airway resistance MCh dose response curves did not show significant differences between the age groups. The results from the present study do not show systematic differences in MCh responsiveness in mice from 2 to 8 wk of age.
Keyword animal models
respiratory impedance
lung volumes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
 
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Created: Wed, 17 Nov 2010, 11:18:50 EST