Stability of interleukin 8 and neutrophil elastase in bronchoalveolar lavage fluid following long-term storage

Berry, Luke J., Sheil, Barbara, Garratt, Luke and Sly, Peter D. (2010) Stability of interleukin 8 and neutrophil elastase in bronchoalveolar lavage fluid following long-term storage. Journal of Cystic Fibrosis, 9 5: 346-350. doi:10.1016/j.jcf.2010.06.002

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Author Berry, Luke J.
Sheil, Barbara
Garratt, Luke
Sly, Peter D.
Title Stability of interleukin 8 and neutrophil elastase in bronchoalveolar lavage fluid following long-term storage
Journal name Journal of Cystic Fibrosis   Check publisher's open access policy
ISSN 1569-1993
1873-5010
Publication date 2010-09
Sub-type Article (original research)
DOI 10.1016/j.jcf.2010.06.002
Volume 9
Issue 5
Start page 346
End page 350
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2011
Language eng
Formatted abstract
Background: Interleukin-8 (IL-8) and neutrophil elastase (NE) are commonly measured markers of inflammation in bronchoalveolar lavage (BAL) fluid from patients with cystic fibrosis. Longitudinal analysis assumes uniform stability during storage, however the effect of extended low-temperature storage on these markers remains unclear.

Methods: BAL fluid from 104 children with cystic fibrosis was assayed for IL-8 and NE after storage at 4 °C for 7 days and − 80 °C for up to 6 years and compared with the initial assays performed soon after collection.

Results: IL-8 levels were stable after any measured length of time at − 80 °C or 4 °C. NE levels were stable for 6 months at − 80 °C but decreased beyond that or after 7 days at 4 °C.

Conclusions: Our data support the stability of IL-8 in BAL stored at − 80 °C for prolonged periods. NE in BAL decreases with storage and should be assayed as soon as practical after collection.

Copyright © 2011 Elsevier B.V.
Keyword Cystic fibrosis
Inflammation
Children
Bronchoalveolar lavage
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Medicine Publications
 
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Created: Wed, 17 Nov 2010, 11:18:18 EST