Repeatability of measuring corneal sub-basal nerve fibre length in individuals with type 2 diabetes

Efron, Nathan, Edwards, Katie, Roper, Nicola, Pritchard, Nicola, Sampson, Geoff P., Shahidi, Ayda M., Vagenas, Dimitrios, Russell, Anthony, Graham, Jim, Dabbah, Mohammad A. and Malik, Rayaz A. (2010) Repeatability of measuring corneal sub-basal nerve fibre length in individuals with type 2 diabetes. Eye and Contact Lens: science and clinical practice, 36 5: 245-248. doi:10.1097/ICL.0b013e3181eea915

Author Efron, Nathan
Edwards, Katie
Roper, Nicola
Pritchard, Nicola
Sampson, Geoff P.
Shahidi, Ayda M.
Vagenas, Dimitrios
Russell, Anthony
Graham, Jim
Dabbah, Mohammad A.
Malik, Rayaz A.
Title Repeatability of measuring corneal sub-basal nerve fibre length in individuals with type 2 diabetes
Journal name Eye and Contact Lens: science and clinical practice   Check publisher's open access policy
ISSN 1542-232;1542-233X
Publication date 2010-09
Sub-type Article (original research)
DOI 10.1097/ICL.0b013e3181eea915
Volume 36
Issue 5
Start page 245
End page 248
Total pages 4
Place of publication Philadelphia, PA, United States
Publisher Lippincott Williams & Wilkins
Collection year 2011
Language eng
Formatted abstract
To analyze the repeatability of measuring nerve fiber length (NFL) from images of the human corneal subbasal nerve plexus using semiautomated software.

Images were captured from the corneas of 50 subjects with type 2 diabetes mellitus who showed varying severity of neuropathy, using the Heidelberg Retina Tomograph 3 with Rostock Corneal Module. Semiautomated nerve analysis software was independently used by two observers to determine NFL from images of the subbasal nerve plexus. This procedure was undertaken on two occasions, 3 days apart.

The intraclass correlation coefficient values were 0.95 (95% confidence intervals: 0.92–0.97) for individual subjects and 0.95 (95% confidence intervals: 0.74–1.00) for observer. Bland-Altman plots of the NFL values indicated a reduced spread of data with lower NFL values. The overall spread of data was less for (a) the observer who was more experienced at analyzing nerve fiber images and (b) the second measurement occasion.

Semiautomated measurement of NFL in the subbasal nerve fiber layer is highly repeatable. Repeatability can be enhanced by using more experienced observers. It may be possible to markedly improve repeatability when measuring this anatomic structure using fully automated image analysis software.

Keyword Corneal nerves
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 40 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 17 Nov 2010, 00:28:12 EST by Dr Anthony Russell on behalf of Medicine - Princess Alexandra Hospital