Wolbachia-Mediated Resistance to Dengue Virus Infection and Death at the Cellular Level

Frentiu, Francesca D., Robinson, Jodie, Young, Paul R., McGraw, Elizabeth A. and O'Neill, Scott L. (2010) Wolbachia-Mediated Resistance to Dengue Virus Infection and Death at the Cellular Level. PLoS One, 5 10 Article # e13398.: 1-8.


Author Frentiu, Francesca D.
Robinson, Jodie
Young, Paul R.
McGraw, Elizabeth A.
O'Neill, Scott L.
Title Wolbachia-Mediated Resistance to Dengue Virus Infection and Death at the Cellular Level
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2010-10
Sub-type Article (original research)
DOI 10.1371/journal.pone.0013398
Volume 5
Issue 10 Article # e13398.
Start page 1
End page 8
Total pages 8
Place of publication San Francisco, CA
Publisher Public Library of Science (PLoS)
Collection year 2011
Language eng
Formatted abstract Background: Dengue is currently the most important arthropod-borne viral disease of humans. Recent work has shown dengue virus displays limited replication in its primary vector, the mosquito Aedes aegypti, when the insect harbors the endosymbiotic bacterium Wolbachia pipientis. Wolbachia-mediated inhibition of virus replication may lead to novel methods of arboviral control, yet the functional and cellular mechanisms that underpin it are unknown.
Methodology/Principal Findings: Using paired Wolbachia-infected and uninfected Aedes-derived cell lines and dengue virus, we confirm the phenomenon of viral inhibition at the cellular level. Although Wolbachia imposes a fitness cost to cells
via reduced proliferation, it also provides a significant degree of protection from virus-induced mortality. The extent of viral inhibition is related to the density of Wolbachia per cell, with highly infected cell lines showing almost complete protection from dengue infection and dramatically reduced virus titers compared to lines not infected with the bacteria.
Conclusions/Significance: We have shown that cells infected with Wolbachia display inhibition of dengue virus replication, that the extent of inhibition is related to bacterial density and that Wolbachia infection, although costly, will provide a fitness benefit in some circumstances. Our results parallel findings in mosquitoes and flies, indicating that cell line models will provide useful and experimentally
© 2010 Frentiu et al.
Keyword Life-shortening Wolbachia
Aedes-aegypti
Mosquito
Host
Drosophila
Strain
Temperature
Albopictus
Pipientis
Symbiont
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Received June 24, 2010; Accepted September 10, 2010; Published October 15, 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biological Sciences Publications
School of Chemistry and Molecular Biosciences
 
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Created: Sun, 07 Nov 2010, 00:03:15 EST