Allelic variation of the β-, γ- and δ-kafirin genes in diverse Sorghum genotypes

Laidlaw, H.K.C., Mace, E.S., Williams, S.B., Sakrewski, K., Mudge, A.M., Prentis, P.J., Jordan, D.R. and Godwin, I.D. (2010) Allelic variation of the β-, γ- and δ-kafirin genes in diverse Sorghum genotypes. Theoretical and Applied Genetics, 121 7: 1227-1237. doi:10.1007/s00122-010-1383-9

Author Laidlaw, H.K.C.
Mace, E.S.
Williams, S.B.
Sakrewski, K.
Mudge, A.M.
Prentis, P.J.
Jordan, D.R.
Godwin, I.D.
Title Allelic variation of the β-, γ- and δ-kafirin genes in diverse Sorghum genotypes
Journal name Theoretical and Applied Genetics   Check publisher's open access policy
ISSN 0040-5752
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1007/s00122-010-1383-9
Volume 121
Issue 7
Start page 1227
End page 1237
Total pages 11
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2011
Language eng
Abstract The β-, γ- and δ-kafirin genes were sequenced from 35 Sorghum genotypes to investigate the allelic diversity of seed storage proteins. A range of grain sorghums, including inbred parents from internationally diverse breeding programs and landraces, and three wild Sorghum relatives were selected to encompass an extensive array of improved and unimproved germplasm in the Eusorghum. A single locus exists for each of the expressed kafirin-encoding genes, unlike the multigenic α-kafirins. Significant diversity was found for each locus, with the cysteine-rich β-kafirin having four alleles, including the first natural null mutant reported for this prolamin subfamily. This allele contains a frame shift insertion at +206 resulting in a premature stop codon. SDS-PAGE revealed that lines with this allele do not produce β-kafirin. An analysis of flour viscosity reveals that these β-kafirin null lines have a difference in grain quality, with significantly lower viscosity observed over the entire Rapid ViscoAnalyser time course. There was less diversity at the protein level within the cysteine-rich γ-kafirin, with only two alleles in the cultivated sorghums. There were only two alleles for the δ-kafirin locus among the S. bicolor germplasm, with one allele encoding ten extra amino acids, of which five were methionine residues, with an additional methionine resulting from a nucleotide substitution. This longer allele encodes a protein with 19.1% methionine. The Asian species, S. propinquum, had distinct alleles for all three kafirin genes. We found no evidence for selection on the three kafirin genes during sorghum domestication even though the δ-kafirin locus displayed comparatively low genetic variation. This study has identified genetic diversity in all single copy seed storage protein genes, including a null mutant for β-kafirin in Sorghum. © 2010 Springer-Verlag.
Keyword In-vitro digestibility
Protein bodies
Maize endosperm
Allele encoding
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
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Created: Sun, 07 Nov 2010, 00:01:11 EST