Fragmentation dynamics of frozen-thawed ram sperm DNA is modulated by sperm concentration

Lopez-Fernandez, C., Johnston, S. D., Fernandez, J. L., Wilson, R. J. and Gosalvez, J. (2010) Fragmentation dynamics of frozen-thawed ram sperm DNA is modulated by sperm concentration. Theriogenology, 74 8: 1362-1370. doi:10.1016/j.theriogenology.2010.06.006


Author Lopez-Fernandez, C.
Johnston, S. D.
Fernandez, J. L.
Wilson, R. J.
Gosalvez, J.
Title Fragmentation dynamics of frozen-thawed ram sperm DNA is modulated by sperm concentration
Journal name Theriogenology   Check publisher's open access policy
ISSN 0093-691X
1879-3231
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1016/j.theriogenology.2010.06.006
Volume 74
Issue 8
Start page 1362
End page 1370
Total pages 9
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2011
Language eng
Formatted abstract
This study investigated the hypothesis that post-thaw incubation of ram sperm at high concentrations results in a faster rate of DNA fragmentation than when sperm are incubated at a lower concentration. Ejaculates from 10 rams were frozen-thawed, prepared in sperm concentrations of 100, 50, 25, 12, and 6 × 106 sperm/mL, and incubated for 6 h at 37 °C. Sperm DNA fragmentation was assessed using the sperm chromatin dispersion test (Sperm-Halomax®) at 1, 3, 4, and 6 h of incubation at 37 °C. On fitting a binary logistic regression with a cubic over time and treating ram and dilution levels as factors, there were significant effects with respect to the ram, dilution and time (all P-values were very much smaller than 0.001). Therefore, DNA fragmentation dynamics of incubated frozen-thawed ram sperm were not only dependent on the inherent sperm DNA fragmentation expressed immediately after thawing, but also on the concentration of sperm incubated in the sample. Although there was evidence of individual ram variation in SDF during the incubation period, the general finding of the current study was that lower sperm concentrations resulted in a slower rate of DNA fragmentation These findings have important implications for the post-thaw manipulation of ram sperm used for AI and advanced reproductive procedures that use sperm at low concentrations. Our data also emphasised the highly dynamic nature of sperm DNA fragmentation and the importance of conducting the procedure in a standardised manner. © 2010 Elsevier Inc.
Keyword Sperm DNA fragmentation
Semen cryopreservation
Sheep
Dilution
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
 
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Created: Sun, 07 Nov 2010, 00:01:00 EST