Improvement in Periodontal Health and Antibody Response to Heat-Shock Proteins

S. Rose-Hill, PJ Ford, SJ Leishman, HL Do, JE Palmer, B Westerman, A Carle, MJ West, GJ Seymour and MP Cullinan (2009). Improvement in Periodontal Health and Antibody Response to Heat-Shock Proteins. In: International Association for Dental Research (IADR) 1st Asia/Pacific Region, Shangri-La Hotel, Wuhan, China, (). 22-24 September, 2009.

Author S. Rose-Hill
PJ Ford
SJ Leishman
JE Palmer
B Westerman
A Carle
MJ West
GJ Seymour
MP Cullinan
Title of paper Improvement in Periodontal Health and Antibody Response to Heat-Shock Proteins
Conference name International Association for Dental Research (IADR) 1st Asia/Pacific Region
Conference location Shangri-La Hotel, Wuhan, China
Conference dates 22-24 September, 2009
Publication Year 2009
Year available 2009
Abstract/Summary There is evidence that periodontal disease may be associated with atherosclerosis due to cross-reactivity of bacterial GroEL immunity with human heat shock protein 60 (hHSP60). Objective: To examine changes in serum antibody responses to Porphyromonas gingivalis, hHSP60, and P. gingivalis GroEL following improvement in periodontal health in patients with cardiovascular disease (CVD) and in patients with high cardiovascular (CV) risk and low CV risk. Methods: Patients were selected from two large longitudinal studies and had undergone yearly periodontal examinations and peripheral blood collections. CVD patients (n=15) selected from one study had experienced a significant CV event while patients derived from the other study had not experienced a CV event. These latter patients were further classified according to CV risk (≥6 classical risk factors=high CV risk n=13; ≤1 classical risk factor=low CV risk n=14). Patients demonstrating a quantifiable improvement in periodontal health (≥62% reduction in number of sites with probing depth≥4mm) from the baseline visit were selected. Serum IgG antibody levels to P.gingivalis, hHSP60, and P.gingivalis GroEL were measured using ELISA. Results: Median reductions, after improvement in periodontal health, in antibody levels to P.gingivalis GroEL differed significantly across the three groups (Kruskal-Wallis p<0.001). These reductions were greatest for high CV-risk patients (908.38ng/mL) compared with 125.18ng/mL for CVD patients, whilst low CV-risk patients showed a slight median increase of 13.57ng/mL. All patients with high CV-risk (13/13=100%) showed reductions in antibody levels to P.gingivalis GroEL compared with CVD (10/15=66.7%) and low-CV risk patients (6/14=42.9%). The difference in these proportions was significant at the 5% level (2 p=0.006). Changes in anti-HSP60 and anti-P.gingivalis antibody levels were not significant. Conclusions: Improved periodontal health reduced levels of the potentially host-reactive antibody, anti- P.gingivalis GroEL, particularly in high CV-risk patients and therefore may be an effective therapy to reduce CV risk for these patients.
Subjects 11 Medical and Health Sciences
110704 Cellular Immunology
Keyword Heat Shock Protein
Cardiovascular disease risk
Porphyromonas gingivalis
P. gingivalis GroEL
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: School of Medicine Publications
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Created: Fri, 05 Nov 2010, 17:32:56 EST by Hong Lien Do on behalf of Faculty Of Health Sciences