An Impedimetric Immunosensor for the Label-Free Detection of Bisphenol A

Rahman, Md. Aminur, Shiddiky, Muhammad J. A., Park, Jang-Su and Shim, Yoon-Bo (2006) An Impedimetric Immunosensor for the Label-Free Detection of Bisphenol A. Biosensors and Bioelectronics, 22 11: 2464-2470. doi:10.1016/j.bios.2006.09.010

Author Rahman, Md. Aminur
Shiddiky, Muhammad J. A.
Park, Jang-Su
Shim, Yoon-Bo
Title An Impedimetric Immunosensor for the Label-Free Detection of Bisphenol A
Journal name Biosensors and Bioelectronics   Check publisher's open access policy
ISSN 0956-5663
Publication date 2006-10-12
Sub-type Article (original research)
DOI 10.1016/j.bios.2006.09.010
Volume 22
Issue 11
Start page 2464
End page 2470
Total pages 7
Editor Anthony P. F. Turner
Place of publication Netherlands
Publisher Elsevier BV
Language eng
Subject C1
Abstract Label-free detection of bisphenolAbased on the impedance measurementwas achieved with an impedimetric immunosensor. The immunosensor was fabricated by the covalent bond formation between a polyclonal antibody and a carboxylic acid group functionalized onto a nano-particle comprised conducting polymer. By using a commercial reagent 4,4-bis(4-hydroxyphenyl) valeric acid (BHPVA), which has an analogous structure of BPA, we have prepared the antigen through the conjugation of BHPVAwith bovine serum albumin (BSA) and then produced a specific polyclonal antibody. The immobilization of antibody and the interaction between antibody and antigen were studied using quartz crystal microbalance (QCM) and electrochemical impedance spectroscopic (EIS) techniques. The impedance and mass changes due to the specific immuno-interaction at the sensor surface were utilized to detect antigen and bisphenol A (BPA). The immunosensor showed specific recognition of BPA with less interference than 4.5% from other common phenolic compounds. Under an optimized condition, the linear dynamic range of BPA detection was between 1 and 100 ng/ml. The detection limit of bisphenol A was determined to be 0.3±0.07 ng/ml. The proposed immunosensor was applied to a human serum sample and the BPA concentration was determined by the standard addition method. © 2006 Elsevier B.V. All rights reserved.
Keyword Bisphenol A
Label-free detection
Polyclonal antibody
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Tue, 02 Nov 2010, 11:23:03 EST by Dr Muhammad J. A. Shiddiky on behalf of Aust Institute for Bioengineering & Nanotechnology