Hydrazine-Catalyzed Ultrasensitive Detection of DNA and Proteins

Shiddiky, Muhammad J. A., Rahman, Md. Aminur and Shim, Yoon-Bo (2007) Hydrazine-Catalyzed Ultrasensitive Detection of DNA and Proteins. ANALYTICAL CHEMISTRY, 79 17: 6886-6890. doi:10.1021/ac0710127

Author Shiddiky, Muhammad J. A.
Rahman, Md. Aminur
Shim, Yoon-Bo
Title Hydrazine-Catalyzed Ultrasensitive Detection of DNA and Proteins
Journal name ANALYTICAL CHEMISTRY   Check publisher's open access policy
ISSN 0003-2700
Publication date 2007-08-08
Sub-type Article (original research)
DOI 10.1021/ac0710127
Volume 79
Issue 17
Start page 6886
End page 6890
Total pages 5
Editor Royce W. Murray
Place of publication United States
Publisher American Chemical Society
Language eng
Subject C1
Abstract A sensitive electrochemical assay of DNA and proteins employing electrocatalytic reduction of hydrogen peroxide by labeled hydrazine on the probe immobilized surfaces was developed. The method utilizes a conducting polymer, poly-5, 2‘:5‘, 2‘‘-terthiophene-3‘-carboxylic acid (pTTCA), covalently linked to the dendrimer (DEN) and hydrazine. The detection signal was amplified by the pTTCA/DEN assembly loaded with Au nanoparticles (particle size, 3.5 nm) onto which huge target DNA- or proteins-linked hydrazine labels (avidin-hydrazine) were adsorbed. The linear dynamic ranges for the electrocatalytic detection of DNA and proteins, extending from 1.0 fM to 10 μM and 10 fg/mL to 10 ng/mL, were observed, along with the detection limits of 450 aM (2700 DNA molecules in a 10-μL sample) and 4.0 fg/mL, respectively. The method eliminates the use of enzymes for DNA and protein detection and opens a way for DNA-free detection of proteins. The simplicity, good reproducibility (RSD, <4.3% for n = 10), and low detection limit of the method offer a good promise for practical DNA and protein analyses.
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 55 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 02 Nov 2010, 11:15:03 EST by Dr Muhammad J. A. Shiddiky on behalf of Aust Institute for Bioengineering & Nanotechnology