Electrophoretic Analysis of Food Dyes Using a Miniaturized Microfluidic System

Lee, Kyung-Sun, Shiddiky, Muhammad J. A., Park, So-Hee, Park, Deok-Su and Shim, Yoon-Bo (2008) Electrophoretic Analysis of Food Dyes Using a Miniaturized Microfluidic System. Electrophoresis, 29 9: 1910-1917. doi:10.1002/elps.200700556

Author Lee, Kyung-Sun
Shiddiky, Muhammad J. A.
Park, So-Hee
Park, Deok-Su
Shim, Yoon-Bo
Title Electrophoretic Analysis of Food Dyes Using a Miniaturized Microfluidic System
Journal name Electrophoresis   Check publisher's open access policy
ISSN 0173-0835
Publication date 2008-04-02
Sub-type Article (original research)
DOI 10.1002/elps.200700556
Volume 29
Issue 9
Start page 1910
End page 1917
Total pages 8
Editor Ziad J. El Rassi
Place of publication Germany
Publisher Wiley - V C H Verlag GmbH & Co
Language eng
Subject C1
Abstract A simple and sensitive on-chip preconcentration, separation, and electrochemical detection (ED) method for the electrophoretic analysis of food dyes was developed. The microchip comprised of three parallel channels: the first two are for the field-amplified sample stacking (FASS) and subsequent field-amplified sample injection (FASI) steps, while the third one is for the micellar EKC with ED (MEKC-ED) step. The food dyes were initially extracted from real samples by employing a method that was simpler, easier, and faster compared with a standard method. The extraction of the samples was characterized by UV–Vis and electrochemical experiments. The chronoamperometric detection was performed with a glassy carbon electrode coupled horizontally with the microchip at the separation channel exit. Experimental parameters affecting the analytical performance of the method were assessed and optimized. The sensitivity of the method was improved by ,10 800-fold when compared with a conventional MEKC-ED analysis. Reproducible response was observed during multiple injections of samples with an RSD of ,7.2% (n = 5). The calibration plots were linear (r2 = 0.998) within the range of 1.0 nM–1.0 mM for all food dyes. LODs were estimated between 1.0 and 5.0 nM, based on S/N = 3, for food dyes. The applicability of the method for the analysis of food dyes in real sample was demonstrated.
Keyword Electrochemical detection
Food dyes
Miceller electrokinetic chromatography
On-chip preconcentration
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 25 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 28 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 02 Nov 2010, 11:11:59 EST by Dr Muhammad J. A. Shiddiky on behalf of Aust Institute for Bioengineering & Nanotechnology