The efficient generation of cell lines from bovine parthenotes

Pashaiasl, M, Khodadadi, K, Holland, MK and Verma, PJ (2010) The efficient generation of cell lines from bovine parthenotes. Cellular Reprogramming, 12 5: 571-579. doi:10.1089/cell.2009.0118

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Author Pashaiasl, M
Khodadadi, K
Holland, MK
Verma, PJ
Title The efficient generation of cell lines from bovine parthenotes
Journal name Cellular Reprogramming   Check publisher's open access policy
ISSN 2152-4971
2152-4998
Publication date 2010-10-11
Sub-type Article (original research)
DOI 10.1089/cell.2009.0118
Volume 12
Issue 5
Start page 571
End page 579
Total pages 9
Place of publication New Rochelle, NY, United States
Publisher Mary Ann Liebert
Collection year 2011
Language eng
Formatted abstract
The generation of embryonic stem cell (ESC) lines from parthenogenetically activated oocytes can provide transplantable cells, which are immunocompatible for the oocyte donors as well as an invaluable tool for genetic engineering and epigenetic studies. We report the efficient isolation of eight putative bovine parthenogenetic embryonic stem cell (bpESC) lines from 15 in vitro produced parthenotes. Five of these cell lines were maintained for more than 15 passages (>140 days) and analyzed. The cells displayed typical ESC morphology, stained positive for alkaline phosphate by histochemical staining, expressed Oct4, Nanog, and either stage-specific embryonic antigens, SSEA1, or SSEA4, detected by immunofluorescence staining. RT-PCR analysis of the cells demonstrated expression of Oct4, Rex1, SSEA1, and ALP. All the cell lines except one had a normal karyotype of 60, XX. The cells differentiated in suspension culture to form embryoid bodies (EBs) expressing markers of the three embryonic germ layers as assessed by RT-PCR. In conclusion, we report efficient derivation of putative ESCs from bovine parthenogenetic embryos. The cells express pluripotent markers, have the ability to form EBs, and differentiate into cells of the three embryonic germ layers. This is the first report of characterized putative parthenogenetic bovine ESC lines. © 2010, Mary Ann Liebert, Inc.
Keyword Embryonic stem-cells
Mouse embryos
Establishment
Culture
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Former titles (until 2010): Cloning and Stem Cells

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 31 Oct 2010, 00:13:06 EST