Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events

Gilding, Edward K. and Marks, M. David (2010) Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events. Plant Journal, 64 2: 304-317.


Author Gilding, Edward K.
Marks, M. David
Title Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Formatted title Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Journal name Plant Journal  (ERA 2012 Listed)    (ERA 2010 Rank A*)   Check publisher's open access policy
Publication date 2010-10
Sub-type Article
DOI 10.1111/j.1365-313X.2010.04329.x
Volume number 64
Issue number 2
ISSN 0960-7412; 1365-313X
Start page 304
End page 317
Total pages 14
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2011
Language eng
Formatted abstract Transcriptome analysis using the Affymetrix ATH1 platform has been completed on purified trichomes from
the gl3-sst mutant. These trichomes display immature features, such as glassy cell walls and blunted branches.
The gl3-sst trichome transcriptome was greatly enriched for genes involved in lipid biosynthesis, including
those mediating the synthesis of fatty acids and wax. In addition, gl3-sst trichomes displayed reduced
expression of the R3 MYBs TRY and CPC, which normally function to limit trichome development. The
expression of the MIXTA-like MYB gene NOK was elevated. Members of the MIXTA-like family promote conical
cell outgrowth, and in some cases, trichome initiation in diverse plant species. In contrast, NOK limits trichome
outgrowth in wild-type Arabidopsis plants. Similar to other MIXTA-like genes, NOK was required for the
expansion of gl3-sst trichomes, as the gl3-sst nok double mutant trichomes were greatly reduced in size.
Expression of NOK in nok mutants reduced branch formation, whereas in gl3-sst nok, NOK expression
promoted trichome cell outgrowth, illustrating duel roles for NOK in both promoting and limiting trichome
development. MIXTA-like genes from phylogenetically diverse plant species could substitute for NOK in both
nok and gl3-sst nok backgrounds. These findings suggest that certain aspects of NOK and MIXTA-like gene
function have been conserved. © 2010 Blackwell Publishing Ltd.
Keyword MIXTA
Transcriptome
ROP
Trichomes
Conical cell
Arabidopsis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
 
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Created: Sun, 31 Oct 2010, 00:03:10 EST