Phosphoinositide 3-kinase δ regulates membrane fission of Golgi carriers for selective cytokine secretion

Low, Pei Ching, Misaki, Ryo, Schroder, Kate, Stanley, Amanda C., Sweet, Matthew J., Teasdale, Rohan D., Vanhaesebroeck, Bart, Meunier, Frédéric A., Taguchi, Tomohiko and Stow, Jennifer L. (2010) Phosphoinositide 3-kinase δ regulates membrane fission of Golgi carriers for selective cytokine secretion. Journal of Cell Biology, 190 6: 1053-1065. doi:10.1083/jcb.201001028

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ219011_OA.pdf Full text (open access) application/pdf 3.27MB 0

Author Low, Pei Ching
Misaki, Ryo
Schroder, Kate
Stanley, Amanda C.
Sweet, Matthew J.
Teasdale, Rohan D.
Vanhaesebroeck, Bart
Meunier, Frédéric A.
Taguchi, Tomohiko
Stow, Jennifer L.
Title Phosphoinositide 3-kinase δ regulates membrane fission of Golgi carriers for selective cytokine secretion
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
1540-8140
Publication date 2010-09-13
Sub-type Article (original research)
DOI 10.1083/jcb.201001028
Open Access Status File (Publisher version)
Volume 190
Issue 6
Start page 1053
End page 1065
Total pages 13
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Collection year 2011
Language eng
Formatted abstract
Phosphoinositide 3-kinase (PI3K) p110 isoforms are membrane lipid kinases classically involved in signal transduction. Lipopolysaccharide (LPS)-activated macrophages constitutively and abundantly secrete proinflammatory cytokines including tumor necrosis factor-α (TNF). Loss of function of the p110δ isoform of PI3K using inhibitors, RNA-mediated knockdown, or genetic inactivation in mice abolishes TNF trafficking and secretion, trapping TNF in tubular carriers at the trans-Golgi network (TGN). Kinase-active p110δ localizes to the Golgi complex in LPS-activated macrophages, and TNF is loaded into p230-labeled tubules, which cannot undergo fission when p110δ is inactivated. Similar blocks in fission of these tubules and in TNF secretion result from inhibition of the guanosine triphosphatase dynamin 2. These findings demonstrate a new function for p110δ as part of the membrane fission machinery required at the TGN for the selective trafficking and secretion of cytokines in macrophages.
Keyword Tumor-necrosis-factor
Protein kinase D
Plasma Membrane
B cell
Phosphatidylinositol 3-kinase
Activated Macrophages
Transport Vesicles
Factor alpha
Network
Complex
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 33 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 24 Oct 2010, 00:12:00 EST