Discovery and distribution of circular peptides in flowering plants

Gruber, C. W., Craik, D. and Kopp, B. (2010). Discovery and distribution of circular peptides in flowering plants. In: EPS 2010: The 31st European Peptide Symposium, Copenhagen, Denmark, (30-30). 5-9 September 2010. doi:10.1002/psc.1301

Author Gruber, C. W.
Craik, D.
Kopp, B.
Title of paper Discovery and distribution of circular peptides in flowering plants
Conference name EPS 2010: The 31st European Peptide Symposium
Conference location Copenhagen, Denmark
Conference dates 5-9 September 2010
Convener European Peptide Society
Journal name Journal of Peptide Science   Check publisher's open access policy
Place of Publication Bognor Regis, West Sussex, U.K.
Publisher John Wiley & Sons
Publication Year 2010
Sub-type Published abstract
DOI 10.1002/psc.1301
ISSN 1075-2617
Volume 16
Issue S1
Start page 30
End page 30
Total pages 1
Collection year 2012
Language eng
Formatted Abstract/Summary
Cyclotides are disulfide-rich miniproteins with the unique structural features of a circular backbone and knotted arrangement of three conserved disulfide bonds. These features make them exceptionally stable and they have applications as host defense (insecticidal) agents and stable drug frameworks. So far they have been found mainly in two plant families, including in every species of the violet family (Violaceae) so far examined, and in a few species of the coffee family (Rubiaceae). Rubiaceae is the fourth largest flowering plant family, comprising approximately 13,000 species, and is one of the largest and most important living biomasses due to their geographical distribution and economic importance.

We analyzed >200 Rubiaceae species and confirmed the presence of cyclotides in 22 species. Additionally, we analyzed >140 species in related plant families to Rubiaceae and Violaceae and for the first time have evidence for the occurrence of cyclotides in the Apocynaceae. On the basis of the phylogeny of cyclotide-bearing plants and the analysis of cyclotide precursor gene sequences, we hypothesize that cyclotide evolution occurred independently in various plant families after the divergence of Asterids and Rosids (~125 mya). This is strongly supported by recent findings on the biosynthesis of cyclotides, which involves ubiquitously present enzymes for folding and processing. In conclusion, we predict that the number of cyclotides within the Rubiaceae may exceed tens of thousands, potentially making cyclotides one of the largest protein families in the plant kingdom.
Q-Index Code EX
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Presented as Oral Presentation O13. Published as Abstract number #137.

Document type: Conference Paper
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
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Created: Sun, 24 Oct 2010, 00:09:46 EST