The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex

Gillespie, Leah K., Hoenen, Antje, Morgan, Gary and Mackenzie, Jason M. (2010) The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex. Journal of Virology, 84 20: 10438-10447. doi:10.1128/JVI.00986-10


Author Gillespie, Leah K.
Hoenen, Antje
Morgan, Gary
Mackenzie, Jason M.
Title The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1070-6321
1098-5514
Publication date 2010-10-01
Sub-type Article (original research)
DOI 10.1128/JVI.00986-10
Open Access Status DOI
Volume 84
Issue 20
Start page 10438
End page 10447
Total pages 10
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2011
Language eng
Formatted abstract
The cytoplasmic replication of positive-sense RNA viruses is associated with a dramatic rearrangement of host cellular membranes. These virus-induced changes result in the induction of vesicular structures that envelop the virus replication complex (RC). In this study, we have extended our previous observations on the intracellular location of West Nile virus strain Kunjin virus (WNVKUN) to show that the virus-induced recruitment of host proteins and membrane appears to occur at a pre-Golgi step. To visualize the WNVKUN replication complex, we performed three-dimensional (3D) modeling on tomograms from WNVKUN replicontransfected cells. These analyses have provided a 3D representation of the replication complex, revealing the open access of the replication complex with the cytoplasm and the fluidity of the complex to the rough endoplasmic reticulum. In addition, we provide data that indicate that a majority of the viral RNA species housed within the RC is in a double-stranded RNA (dsRNA) form. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Keyword Infected cells
Brome mosaic-virus
Rubella-virus
Dengue virus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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Created: Sun, 24 Oct 2010, 10:07:26 EST