Subfractionation of differentiating human embryonic stem cell populations allows the isolation of a mesodermal population enriched for intermediate mesoderm and putative renal progenitors

Lin, S. Adelia, Kolle, Gabriel, Grimmond, Sean M., Zhou, Qi, Doust, Elizabeth, Little, Melissa H., Aronow, Bruce, Ricardo, Sharon D., Pera, Martin F., Bertram, John F. and Laslett, Andrew L. (2010) Subfractionation of differentiating human embryonic stem cell populations allows the isolation of a mesodermal population enriched for intermediate mesoderm and putative renal progenitors. Stem Cells and Development, 19 10: 1637-1648. doi:10.1089/scd.2010.0017


Author Lin, S. Adelia
Kolle, Gabriel
Grimmond, Sean M.
Zhou, Qi
Doust, Elizabeth
Little, Melissa H.
Aronow, Bruce
Ricardo, Sharon D.
Pera, Martin F.
Bertram, John F.
Laslett, Andrew L.
Title Subfractionation of differentiating human embryonic stem cell populations allows the isolation of a mesodermal population enriched for intermediate mesoderm and putative renal progenitors
Journal name Stem Cells and Development   Check publisher's open access policy
ISSN 1547-3287
1557-8534
Publication date 2010-10
Sub-type Article (original research)
DOI 10.1089/scd.2010.0017
Volume 19
Issue 10
Start page 1637
End page 1648
Total pages 12
Place of publication Larchmont, NY, U.S.
Publisher Mary Ann Liebert
Collection year 2011
Language eng
Formatted abstract
Human embryonic stem (ES) cells are pluripotent and are believed to be able to generate all cell types in the body. As such, they have potential applications in regenerative therapy for kidney disease. However, before this can be achieved, a protocol to differentiate human ES cells to mesodermal renal progenitor lineages is required. Reduction of serum concentration and feeder layer density reduction cultures were used to differentiate human ES cells for 14 days. Differentiated ES cells were then fractionated by flow cytometry based on expression of the markers CD24, podocalyxin, and GCTM2 to isolate putative renal cells. These cells up-regulated the expression of the renal transcription factors PAX2, LHX1, and WT1 when compared with unfractionated human ES cells. Immunohistochemical assays confirmed that a subset of cells within this fraction co-expressed nuclear WT1 and PAX2 proteins. Transcriptome profiling also showed that the most differentially up-regulated genes in this fraction preferentially associated with kidney development in comparison with any other lineage. When compared with a transcriptome profile database of urogenital development (GUDMAP), the top 200 differentially up-regulated genes in this fraction strongly clustered into a group of genes associated with the metanephric mesenchyme at E11.5 and the corticonephrogenic interstitium at E15.5 of murine kidney development. Hence, this approach confirms an ability to direct human ES cells toward a renal progenitor state.
© 2010, Mary Ann Liebert, Inc.
Keyword In-vitro
Directed Differentiation
Definitive Endoderm
Human Blastocysts
Germ-cells
Kidney
Precursors
Mouse
Lines
Trophoblast
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Received for publication January 12, 2010 Accepted after revision February 9, 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Institute for Molecular Bioscience - Publications
 
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Created: Sun, 24 Oct 2010, 00:01:57 EST