The construction, fouling and enzymatic cleaning of a textile dye surface

Onaizi, Sagheer A., He, Lizhong and Middelberg, Anton P.J. (2010) The construction, fouling and enzymatic cleaning of a textile dye surface. Journal of Colloid and Interface Science, 351 1: 203-209. doi:10.1016/j.jcis.2010.07.030


Author Onaizi, Sagheer A.
He, Lizhong
Middelberg, Anton P.J.
Title The construction, fouling and enzymatic cleaning of a textile dye surface
Journal name Journal of Colloid and Interface Science   Check publisher's open access policy
ISSN 0021-9797
1095-7103
Publication date 2010-11
Sub-type Article (original research)
DOI 10.1016/j.jcis.2010.07.030
Volume 351
Issue 1
Start page 203
End page 209
Total pages 7
Place of publication Maryland Heights, Missouri, U.S.A.
Publisher Academic Press
Collection year 2011
Language eng
Subject C1
0904 Chemical Engineering
Abstract The enzymatic cleaning of a rubisco protein stain bound onto Surface Plasmon Resonance (SPR) biosensor chips having a dye-bound upper layer is investigated. This novel method allowed, for the first time, a detailed kinetic study of rubisco cleanability (defined as fraction of adsorbed protein removed from a surface) from dyed surfaces (mimicking fabrics) at different enzyme concentrations. Analysis of kinetic data using an established mathematical model able to decouple enzyme transfer and reaction processes [Onaizi, He, Middelberg, Chem. Eng. Sci. 64 (2008) 3868] revealed a striking effect of dyeing on enzymatic cleaning performance. Specifically, the absolute rate constants for enzyme transfer to and from a dye-bound rubisco stain were significantly higher than reported previously for un-dyed surfaces. These increased transfer rates resulted in higher surface cleanability. Higher enzyme mobility (i.e., higher enzyme adsorption and desorption rates) at the liquid–dye interface was observed, consistent with previous suggestions that enzyme surface mobility is likely correlated with overall enzyme cleaning performance. Our results show that reaction engineering models of enzymatic action at surfaces may provide insight able to guide the design of better stain-resistant surfaces, and may also guide efforts to improve cleaning formulations. © 2010 Elsevier Inc.
Keyword Dye
Surface
Biosensor
Proteolysis
Fouling
Rubisco
Cleaning
Subtilisin
Adsorption
Modelling
Self-assembled Monolayers
Cibacron blue F3GA
NAD(H) Dependent lactate dehydrogenase
Model cellulose films
Biospecific affinity
Albumin adsorption
Protein adsorption
Lateral Diffusion
Human Plasma
Lysozyme adsorption
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Sun, 17 Oct 2010, 00:07:37 EST