"Tips and tricks" for high-pressure freezing of model systems

McDonald, K, Schwarz, H, Muller-Reichert, T, Webb, R, Buser, C and Morphew, M (2010) "Tips and tricks" for high-pressure freezing of model systems. Methods in Cell Biology, 96 C: 671-693. doi:10.1016/S0091-679X(10)96028-7

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Author McDonald, K
Schwarz, H
Muller-Reichert, T
Webb, R
Buser, C
Morphew, M
Title "Tips and tricks" for high-pressure freezing of model systems
Journal name Methods in Cell Biology   Check publisher's open access policy
ISSN 0091-679X
ISBN 978-0-12-381007-6
Publication date 2010
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/S0091-679X(10)96028-7
Volume 96
Issue C
Start page 671
End page 693
Total pages 23
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2011
Language eng
Abstract High-pressure freezing (HPF) has been around since the mid-1980s as a cryopreparation technique for biological electron microscopy. It has taken quite some time to "catch on" but with the recent interest in cellular tomography and electron microscopy of vitreous cryosections it has been used more frequently. While HPF is relatively easy to do, there are a number of steps, such as loading the sample into the specimen carrier correctly, that are critical to the success of this method. In this chapter we discuss some of the "little" things that can make the difference between successful or unsuccessful freezing. We cover all aspects of HPF, from specimen loading to removing your sample from the carriers in polymerized resin. Our goal is to make it easier and more reliable for HPF users to get well-frozen samples for their research. © 2010 Elsevier Inc.
Keyword Electron-microscopy
Correlative light
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Volume 'Electron Microscopy of Model Systems' Edited by: Thomas Müller-Reichert. Chapter 28.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2011 Collection
Centre for Microscopy and Microanalysis Publications
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Citation counts: TR Web of Science Citation Count  Cited 32 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 33 times in Scopus Article | Citations
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Created: Sun, 10 Oct 2010, 00:05:41 EST