Dynamic isomiR regulation in Drosophila development

Fernandez-Valverde, Selene L., Taft, Ryan J. and Mattick, John S. (2010) Dynamic isomiR regulation in Drosophila development. RNA, 16 10: 1881-1888. doi:10.1261/rna.2379610

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Author Fernandez-Valverde, Selene L.
Taft, Ryan J.
Mattick, John S.
Title Dynamic isomiR regulation in Drosophila development
Journal name RNA   Check publisher's open access policy
ISSN 1355-8382
1469-9001
Publication date 2010-10-01
Sub-type Article (original research)
DOI 10.1261/rna.2379610
Open Access Status File (Publisher version)
Volume 16
Issue 10
Start page 1881
End page 1888
Total pages 8
Place of publication Woodbury, NY, United States
Publisher Cold Spring Harbor Laboratory Press
Collection year 2011
Language eng
Formatted abstract
Several recent reports have demonstrated that microRNAs (miRNAs) can exhibit heterogeneous ends and post-transcriptional nontemplate 3′ end additions of uridines or adenosines. Using two small RNA deep-sequencing data sets, we show here that these miRNA isoforms (isomiRs) are differentially expressed across Drosophila melanogaster development and tissues. Specifically, we demonstrate that: (1) nontemplate nucleotide additions of adenosines to miRNA 3′ ends are highly abundant in early development; (2) a subset of miRNAs with nontemplate 3′ Us are expressed in adult tissues; and (3) the size of at least eight "mature" (unmodified) miRNAs varies in a life-cycle or tissue-specific manner. These results suggest that subtle variability in isomiR expression, which is widely thought to be the result of inexact Dicer processing, is regulated and biologically meaningful. Indeed, a subset of the miRNAs enriched for 3′ adenosine additions during early embryonic development, including miR-282 and miR-312, show enrichment for target sites in developmental genes that are expressed during late embryogenesis, suggesting that nontemplate additions increase miRNA stability or strengthen miRNA:target interactions. This work suggests that isomiR expression is an important aspect of miRNA biology, which warrants further investigation.
Copyright © 2010 RNA Society.
Keyword microRNA
GLD-2
Adenylation
Embryogenesis
miR-282
miR-312
miR-8
Cytoplasmic Polyadenylation
Poly(a) Polymerase
Target recognition
Gene-expression
Micrornas
Embryogenesis
Uridylation
Adenylation
Biogenesis
Mechanisms
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published in Advance August 30, 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Institute for Molecular Bioscience - Publications
 
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Created: Sun, 10 Oct 2010, 10:00:51 EST