Use of GFP to analyze morphology, connectivity, and function of cells in the central nervous system

Harvey, Alan R., Ehlert, Erich, de Wit, Joris, Drummond, Eleanor S., Pollett, Margaret A., Ruitenberg, Marc J., Plant, Giles W., Verhaagen, Joost and Levelt, Christiaan N. (2009). Use of GFP to analyze morphology, connectivity, and function of cells in the central nervous system. In Barry W. Hicks (Ed.), Viral Applications of Green Fluorescent Protein: Methods and Protocols (pp. 63-95) New York, USA: Humana. doi:10.1007/978-1-59745-559-6_5


Author Harvey, Alan R.
Ehlert, Erich
de Wit, Joris
Drummond, Eleanor S.
Pollett, Margaret A.
Ruitenberg, Marc J.
Plant, Giles W.
Verhaagen, Joost
Levelt, Christiaan N.
Title of chapter Use of GFP to analyze morphology, connectivity, and function of cells in the central nervous system
Title of book Viral Applications of Green Fluorescent Protein: Methods and Protocols
Place of Publication New York, USA
Publisher Humana
Publication Year 2009
Sub-type Other
DOI 10.1007/978-1-59745-559-6_5
Series Methods in Molecular Biology
ISBN 9781934115879
ISSN 1064-3745
Editor Barry W. Hicks
Volume number 515
Chapter number 5
Start page 63
End page 95
Total pages 33
Total chapters 24
Language eng
Subjects 0601 Biochemistry and Cell Biology
Abstract/Summary We here describe various approaches using GFP that are being used in the morphological and functional analysis of specific cell types in the normal and injured central nervous system. Incorporation of GFP into viral vectors allows phenotypic characterization of transduced cells and can be used to label their axons and terminal projections. Characterization of transduced cell morphology can be enhanced by intracellular injection of living GFP-labeled cells with appropriate fluorescent dyes. Ex vivo labeling of precursor or glial cells using viral vectors that encode GFP permits long-term identification of these cells after transplantation into the brain or spinal cord. In utero electroporation methods result in expression of gene products in developing animals, allowing both functional and morphological studies to be carried out. GFPCre has been developed as a marker gene for viral vector-mediated expression of the bacterial recombinase Cre in the brain of adult mice with “floxed” transgenes. GFPCre-mediated induction of transgene expression can be monitored by GFP expression in defined populations of neurons in the adult brain. Finally, GFP can be used to tag proteins, permitting dynamic visualization of the protein of interest in living cells.
Keyword Viral vectors
Adeno-associated virus
Lentivirus
Electroporation
Olfactory ensheathing glia
Schwann cells
Cre-EGFP
Retina
Semaphorin
Hippocampus
Neocortex
Q-Index Code BX
Q-Index Status Provisional Code

Document type: Book Chapter
Collection: School of Biomedical Sciences Publications
 
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Created: Wed, 06 Oct 2010, 11:37:59 EST by Laura McTaggart on behalf of Faculty of Science