Recycling endosome membrane incorporation into the leading edge regulates lamellipodia formation and macrophage migration

Veale, Kelly J., Offenhäuser, Carolin, Whittaker, Shane P., Estrella, Ruby P. and Murray, Rachael Z. (2010) Recycling endosome membrane incorporation into the leading edge regulates lamellipodia formation and macrophage migration. Traffic, 11 10: 1370-1379. doi:10.1111/j.1600-0854.2010.01094.x


Author Veale, Kelly J.
Offenhäuser, Carolin
Whittaker, Shane P.
Estrella, Ruby P.
Murray, Rachael Z.
Title Recycling endosome membrane incorporation into the leading edge regulates lamellipodia formation and macrophage migration
Journal name Traffic   Check publisher's open access policy
ISSN 1398-9219
1600-0854
Publication date 2010-10
Sub-type Article (original research)
DOI 10.1111/j.1600-0854.2010.01094.x
Volume 11
Issue 10
Start page 1370
End page 1379
Total pages 10
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell Publishing
Collection year 2011
Language eng
Abstract In comparison to our knowledge of the recycling of adhesion receptors and actin assembly, exactly how the cell controls its surface membrane to form a lamellipodium during migration is poorly understood. Here, we show the recycling endosome membrane is incorporated into the leading edge of a migrating cell to expand lamellipodia membrane. We have identified the SNARE complex that is necessary for fusion of the recycling endosome with the cell surface, as consisting of the R-SNARE VAMP3 on the recycling endosome partnering with the surface Q-SNARE Stx4/SNAP23, which was found to translocate and accumulate on the leading edge of migrating cells. Increasing VAMP3-mediated fusion of the recycling endosome with the surface increased membrane ruffling, while inhibition of VAMP3-mediated fusion showed that incorporation of the recycling endosome is necessary for efficient lamellipodia formation. At the same time, insertion of this recycling endosome membrane also delivers its cargo integrin α5β1 to the cell surface. The loss of this extra membrane for lamellipodia expansion and delivery of cargo in cells resulted in macrophages with a diminished capacity to effectively migrate. Thus, the recycling endosome membrane is incorporated into the leading edge and this aids expansion of the lamellipodia and simultaneously delivers integrins necessary for efficient cell migration. © 2010 John Wiley & Sons A/S.
Keyword Integrin
Membrane fusion
Recycling endosome and migration
SNARE
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 26 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 29 times in Scopus Article | Citations
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Created: Sun, 03 Oct 2010, 00:00:45 EST