The prevalence of embryonic remnants following the recovery of post-hatching bovine embryos produced in vitro or by somatic cell nuclear transfer

Alexopoulos, Natalie I. and French, Andrew J. (2009) The prevalence of embryonic remnants following the recovery of post-hatching bovine embryos produced in vitro or by somatic cell nuclear transfer. Animal Reproduction Science, 114 1-3: 43-53. doi:10.1016/j.anireprosci.2008.09.010


Author Alexopoulos, Natalie I.
French, Andrew J.
Title The prevalence of embryonic remnants following the recovery of post-hatching bovine embryos produced in vitro or by somatic cell nuclear transfer
Journal name Animal Reproduction Science   Check publisher's open access policy
ISSN 0378-4320
Publication date 2009-08-01
Sub-type Article (original research)
DOI 10.1016/j.anireprosci.2008.09.010
Volume 114
Issue 1-3
Start page 43
End page 53
Total pages 11
Place of publication The Netherlands
Publisher Elsevier B.V.
Language eng
Subject 0707 Veterinary Sciences
0702 Animal Production
Abstract The reliable collection of peri-implantation embryos in the bovine has important ramifications to post-transfer consequences, particularly in the elucidation of mechanisms associated with post-hatching embryo development and to perturbations in developmental growth following transfer. This study analyzed both in vitro produced (IVP) and somatic cell nuclear transfer (SCNT) embryo-like structures (ELS) recovered at Day (D) 14 and D21. The recovered ELS were subsequently processed for histological examination. At D14 and D21, many of the embryos recovered in the IVP group conformed to the appropriate stage of development. However, a significant number of anomalies were present in the SCNT groups when examined in more detail. Histological examination revealed that irrespective of whether these embryos had undergone trophoblast expansion to an ovoid, tubular or filamentous morphology, many had a degenerated hypoblast layer and a large proportion did not possess an epiblast and therefore could not differentiate into any of the three germ layers as would be expected at the neural groove or somite stage. The prevalence of this developmental pattern was random and did not correlate with treatment (IVP or SCNT) or with types of structures recovered. The rapid embryo elongation period also coincides with the time of greatest embryonic loss and these observations could have important implications for assessing the recovery of embryos post-transfer where incorrect morphological assessment could lead to false implantation and pregnancy determination rates. The implementation of additional methodology is required to adequately characterize the quality of IVP and SCNT-derived embryos collected post-transfer.
Keyword Embryonic remnants
Hypoblast
Epiblast
Germ layer
Recovery rate
Embryo loss
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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Created: Wed, 29 Sep 2010, 19:58:18 EST by Laura McTaggart on behalf of Queensland Brain Institute