Towards the immunoproteome of Neisseria meningitidis

Mendum, Tom A., Newcombe, Jane, McNeilly, Celia L. and McFadden, Johnjoe (2009) Towards the immunoproteome of Neisseria meningitidis. PLoS One, 4 6: . doi:10.1371/journal.pone.0005940

Author Mendum, Tom A.
Newcombe, Jane
McNeilly, Celia L.
McFadden, Johnjoe
Title Towards the immunoproteome of Neisseria meningitidis
Formatted title
Towards the Immunoproteome of Neisseria meningitidis
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2009-06-16
Sub-type Article (original research)
DOI 10.1371/journal.pone.0005940
Open Access Status DOI
Volume 4
Issue 6
Total pages 12
Place of publication United States
Publisher Public Library of Science
Language eng
Subject 10 Technology
09 Engineering
11 Medical and Health Sciences
Formatted abstract
Despite the introduction of conjugated polysaccharide vaccines for many of the Neisseria meningitidis serogroups, neisserial infections continue to cause septicaemia and meningitis across the world. This is in part due to the difficulties in developing a, cross-protective vaccine that is effective against all serogroups, including serogroup B meningococci. Although convalescent N. meningitidis patients develop a natural long-lasting cross-protective immunity, the antigens that mediate this response remain unknown. To help define the target of this protective immunity we identified the proteins recognized by IgG in sera from meningococcal patients by a combination of 2D protein gels, western blots and mass spectrometry. Although a number of outer membrane antigens were identified the majority of the antigens were cytoplasmic, with roles in cellular processes and metabolism. When recombinant proteins were expressed and used to raise sera in mice, none of the antigens elicited a positive SBA result, however flow cytometry did demonstrate that some, including the ribosomal protein, RplY were localised to the neisserial cell surface.
Q-Index Code C1
Q-Index Status Provisional Code
Additional Notes Article number e5940

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 28 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 28 Sep 2010, 12:29:20 EST by Laura McTaggart on behalf of Aust Institute for Bioengineering & Nanotechnology