Functional disulfide-stabilized polymer-protein particles

Jia, Zhongfan, Liu, Jingquan, Boyer, Cyrille, Davis, Thomas P. and Bulmus, Volga (2009) Functional disulfide-stabilized polymer-protein particles. Biomacromolecules, 10 12: 3253-3258. doi:10.1021/bm900817a


Author Jia, Zhongfan
Liu, Jingquan
Boyer, Cyrille
Davis, Thomas P.
Bulmus, Volga
Title Functional disulfide-stabilized polymer-protein particles
Formatted title
Functional disulfide-stabilized polymer–protein particles
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1525-7797
1526-4602
Publication date 2009-12-14
Sub-type Article (original research)
DOI 10.1021/bm900817a
Volume 10
Issue 12
Start page 3253
End page 3258
Total pages 6
Place of publication Washington, DC, U.S.A.
Publisher American Chemical Society
Language eng
Subject 03 Chemical Sciences
06 Biological Sciences
09 Engineering
Formatted abstract
Polymer - protein hybrid particles (PPHPs) have a significant potential in drug delivery, diagnosis, and biomedical imaging applications. Herein, we describe a simple route to disulfide cross-linked, poly(ethylene glycol)-streptavidin hybrid particles with tunable diameters. These particles have great versatility and potential for a number of reasons. First, they possess free biotin binding sites on their streptavidin (SAv) coated surface, enabling the conjugation of any biotinylated-molecule such as biotinylated antibodies. Second, core-stabilization can easily be controlled using reversible disulfide cross-links, and third, thiol- and ene-reactive functionalities in the core are available for the conjugation of drugs and labels. In detail, micelles having a biotinylated poly(ethylene glycol) corona and a disulfide cross-linked, reactive core were formed using α-biotin PEG-b-poly(pyridyldisulfide ethylmethacrylate) block copolymers synthesized via RAFT polymerization. Functionalization of the micelle core was performed in a one-pot reaction concurrent with the micellization and cross-linking processes by using a thiol-reactive model compound (a maleimide derivative of a green fluorophore). The resultant micelles displayed spherical morphology with a diameter of 54 ± 4 nm. Biotin functionality was largely exposed on the micelle corona (75 mol % availability), as determined by a streptavidin/HABA assay. The micelles were subsequently decorated with (red fluorophore-labeled) streptavidin (SAv) through the accessible biotins on the surface, yielding SAv-linked micelle aggregates with tunable dimensions (in the range between 350 nm and 2 μm), as determined by transmission electron microscopy. Fluorescent-labels on the particles were monitored using confocal microscopy, revealing that the SAv coats the periphery of the PPHPs.
© 2009 American Chemical Society
Keyword RAFT polymerization
Responsive polymer
Nanoparticles
Streptavidin
Delivery
Copolymers
Binding
Agent
ATRP
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Mon, 27 Sep 2010, 15:17:18 EST by Jon Swabey on behalf of Aust Institute for Bioengineering & Nanotechnology