Exocytosis, dependent on Ca2+ release from Ca2+ stores, is regulated by Ca2+ microdomains

Low, Jiun T., Shukla, Alka, Behrendorff, Natasha and Thorn, Peter (2010) Exocytosis, dependent on Ca2+ release from Ca2+ stores, is regulated by Ca2+ microdomains. Journal of Cell Science, 123 18: 3201-3208. doi:10.1242/jcs.071225

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Author Low, Jiun T.
Shukla, Alka
Behrendorff, Natasha
Thorn, Peter
Title Exocytosis, dependent on Ca2+ release from Ca2+ stores, is regulated by Ca2+ microdomains
Formatted title
Exocytosis, dependent on Ca2+ release from Ca2+ stores, is regulated by Ca2+ microdomains
Journal name Journal of Cell Science   Check publisher's open access policy
ISSN 0021-9533
1477-9137
Publication date 2010-09
Sub-type Article (original research)
DOI 10.1242/jcs.071225
Open Access Status File (Publisher version)
Volume 123
Issue 18
Start page 3201
End page 3208
Total pages 8
Editor Fiona M Watt
Richard Sever
Place of publication Cambridge, U.K.
Publisher Company of Biologists
Collection year 2011
Language eng
Subject C1
0601 Biochemistry and Cell Biology
Formatted abstract
The relationship between the cellular Ca2+ signal and secretory vesicle fusion (exocytosis) is a key determinant of the regulation of the kinetics and magnitude of the secretory response. Here, we have investigated secretion in cells where the exocytic response is controlled by Ca2+ release from intracellular Ca2+ stores. Using live-cell two-photon microscopy that simultaneously records Ca2+ signals and exocytic responses, we provide evidence that secretion is controlled by changes in Ca2+ concentration [Ca2+] in relatively large-volume microdomains. Our evidence includes: (1) long latencies (>2 seconds) between the rise in [Ca2+] and exocytosis, (2) observation of exocytosis all along the lumen and not clustered around Ca2+ release hot-spots, (3) high affinity (Kd=1.75 μM) Ca2+ dependence of exocytosis, (4) significant reduction in exocytosis in the prescence of cytosolic EGTA, (5) spatial exclusion of secretory granules from the cell membrane by the endoplasmic reticulum, and (6) inability of local Ca 2+ responses to trigger exocytosis. These results strongly indicate that the control of exocytosis, triggered by Ca2+ release from stores, is through the regulation of cytosolic [Ca2+] within a microdomain.
© 2010. Published by The Company of Biologists Ltd.

Keyword Exocytosis
Calcium
Epithelial
Zymogen
Pancreatic
Two photon
Pancreatic Acinar-Cells
Inositol 1,4,5-Triphosphate receptors
Zymogen Granule Membranes
Exocrine cells
Endoplasmic-reticulum
Polarised expression
Transitter release
Chromaffin cells
Amylase release
Xenopus-oocytes
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biomedical Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 16 times in Scopus Article | Citations
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Created: Sun, 26 Sep 2010, 00:04:13 EST