Subcellular localization of the MNV-1 ORF1 proteins and their potential roles in the formation of the MNV-1 replication complex

Hyde, Jennifer L. and Mackenzie, Jason M. (2010) Subcellular localization of the MNV-1 ORF1 proteins and their potential roles in the formation of the MNV-1 replication complex. Virology, 406 1: 138-148. doi:10.1016/j.virol.2010.06.047


Author Hyde, Jennifer L.
Mackenzie, Jason M.
Title Subcellular localization of the MNV-1 ORF1 proteins and their potential roles in the formation of the MNV-1 replication complex
Journal name Virology   Check publisher's open access policy
ISSN 0042-6822
1089-862X
1096-0341
Publication date 2010-10
Sub-type Article (original research)
DOI 10.1016/j.virol.2010.06.047
Volume 406
Issue 1
Start page 138
End page 148
Total pages 11
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2011
Language eng
Abstract Human noroviruses are the leading cause of nonbacterial gastroenteritis worldwide and are now recognised as a significant human pathogen. Whereas human noroviruses cannot be cultivated in the laboratory, mouse norovirus 1 (MNV-1) is easily cultivated and has a defined tropism for cells of a mononuclear origin. As such, MNV-1 provides an ideal opportunity to study many aspects of norovirus biology and replication. Previously, we have shown that MNV-1 RNA replication is associated with components of the early and late secretory pathway and that all six open reading frame 1 (ORF1) proteins are associated with the viral dsRNA within the replication complex (RC) during the course of infection. In this study, we further characterise the subcellular localisation of the MNV-1 ORF1 proteins when recombinantly expressed in cells. We show that two MNV-1 proteins, NS1-2 and NS4, associate with the endoplasmic reticulum and endosomes, respectively. Whereas NS6 (the viral protease) appeared to localize within the cytoplasm and to mitochondria, NS7 (the viral polymerase) was observed to localize diffusely within the cytoplasm and within the nucleus, and NS3 localized to discrete foci within the cytoplasm which were of unknown origin. Based on the localization patterns observed we propose a model by which NS1-2 and NS4 may recruit host membranes to the MNV-1 RC during replication. © 2010 Elsevier Inc.
Keyword Mouse norovirus
Virus replication
Membrane induction
Mouth-disease virus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Sun, 26 Sep 2010, 00:00:23 EST