Molecular identification of weedy Sporobolus species by PCR-RFLP

Shrestha, S., Graham, G. C., Loch, D. S. and Adkins, S. W. (2010) Molecular identification of weedy Sporobolus species by PCR-RFLP. Weed Research, 50 5: 383-387. doi:10.1111/j.1365-3180.2010.00806.x

Author Shrestha, S.
Graham, G. C.
Loch, D. S.
Adkins, S. W.
Title Molecular identification of weedy Sporobolus species by PCR-RFLP
Journal name Weed Research   Check publisher's open access policy
ISSN 0043-1737
Publication date 2010-10
Sub-type Article (original research)
DOI 10.1111/j.1365-3180.2010.00806.x
Volume 50
Issue 5
Start page 383
End page 387
Total pages 5
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2011
Language eng
Formatted abstract
Identification of the five noxious weedy Sporobolus species from non-weedy and native species in Australia is extremely difficult. Furthermore, seeds of these species, which are found in commercial seed lots, are morphologically indistinguishable. The lack of a reliable identification protocol is contributing to the rapid spread of these noxious weedy species within Australia. A polymerase chain reaction-restriction fragment length polymorphism approach carried out on nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS) sequences was used to create a molecular diagnostic tool. To develop this tool, 40 ITS sequences generated from 14 Sporobolus species were used. A restriction enzyme (MvnI) was identified which could cut the ITS sequences from the noxious weedy species into two approximately equal fragments, while not cutting those from all other species. When tested on 23 randomly selected seed samples, identification was accomplished with 100% accuracy, suggesting a possible application in seed certification programmes.
© 2010 The Authors. Weed Research © 2010 European Weed

Keyword Diagnostics
Giant rats tail grasses
Internal transcribed spacers (ITS)
Polymerase chain reaction
Ribosomal DNA
ITS sequences
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Official 2011 Collection
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 1 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 26 Sep 2010, 00:00:10 EST