Synthesis and characterization of DNA-based micro- and nanodumbbell structures

Rahaie, Mahdi, Ghai, Rajesh, Babić, Bakir and Dimitrov, Krassen (2009) Synthesis and characterization of DNA-based micro- and nanodumbbell structures. Journal of Bionanoscience, 3 2: 73-79. doi:10.1166/jbns.2009.1010

Author Rahaie, Mahdi
Ghai, Rajesh
Babić, Bakir
Dimitrov, Krassen
Title Synthesis and characterization of DNA-based micro- and nanodumbbell structures
Journal name Journal of Bionanoscience   Check publisher's open access policy
ISSN 1557-7910
Publication date 2009-12
Sub-type Article (original research)
DOI 10.1166/jbns.2009.1010
Volume 3
Issue 2
Start page 73
End page 79
Total pages 8
Place of publication United States
Publisher American Scientific Publishers
Language eng
Subject 10 Technology
09 Engineering
Abstract Static barcode labels for individual molecules, based on a sequence of fluorescent segments deterministically encoded in a DNA scaffold, are emerging as sensitive tools for direct digital quantification assays, such as RNA transcript profiling. The next generation of digital barcoding will require dynamic single-molecule labels, which can be controllably translocated, stretched, and read serially by an electronic or optical point detector. Towards that goal, structures consisting of a single magnetic and a single non-magnetic particle connected by a DNA-based linker were synthesized at micron (microdumbbells) and submicron (nanodumbells) scales via successive streptavidin-biotin attachments. Visualization of the structures with fluorescent and atomic force microscopy revealed the desired dumbbell morphology, thus demonstrating the suitability of the synthesis scheme. The stretched DNA-based linker is a convenient template for deterministic, sequence specific information encoding, which indicates the potential of the dumbbell-shaped nanostructures as magnetophoretically driven dynamic barcode labels.
Keyword DNA Labelling
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Thu, 23 Sep 2010, 15:02:50 EST by Laura McTaggart on behalf of Aust Institute for Bioengineering & Nanotechnology