Human alloantibody anti-Mart interferes with Mac-1-dependent leukocyte adhesion

Sachs, Ulrich J. H., Chavakis, Sachs, Triantafyllos, Fung, Lin, Lohrenz, Alexander, Bux, Jürgen, Reil, Angelika, Ruf, Andreas and Santoso, Sentot (2004) Human alloantibody anti-Mart interferes with Mac-1-dependent leukocyte adhesion. Blood, 104 3: 727-734. doi:10.1182/blood-2003-11-3809


Author Sachs, Ulrich J. H.
Chavakis, Sachs, Triantafyllos
Fung, Lin
Lohrenz, Alexander
Bux, Jürgen
Reil, Angelika
Ruf, Andreas
Santoso, Sentot
Title Human alloantibody anti-Mart interferes with Mac-1-dependent leukocyte adhesion
Journal name Blood   Check publisher's open access policy
ISSN 0006-4971
1528-0020
Publication date 2004-08
Sub-type Article (original research)
DOI 10.1182/blood-2003-11-3809
Volume 104
Issue 3
Start page 727
End page 734
Total pages 8
Place of publication Washington, DC, United States
Publisher American Society of Hematology
Language eng
Abstract The CD11b/CD18 integrin plays a crucial role in cell-cell adhesion processes. Recently, we described a case of severe neonatal alloimmune neutropenia (NAIN) caused by an alloantibody against a variant of the CD11b subunit (Mart alloantigen). Allele-specific transfected cells allowed us to demonstrate that an H61R point mutation is directly responsible for the formation of Mart epitopes. No difference in the adhesion capability between H61 and R61 homozygous neutrophils was observed. Functional analysis showed that anti-Mart inhibited Mac-1-dependent adhesion of neutrophils and monocytic U937 cells to fibrinogen, intercellular adhesion molecule-1 (ICAM-1), receptor for advanced glycation and product (RAGE), and glycoprotein Ibα but not to junctional adhesion molecule-C or urokinase plasminogen activator receptor (uPAR). Accordingly, anti-Mart blocked neutrophil and U937 cell adhesion to endothelial cells and platelet-leukocyte aggregate formation in whole blood under high shear. Other sera of anti-Mart from mothers of infants without NAIN did not show inhibitory properties. We conclude that anti-Mart antibodies with different functional properties exist. This is supported by our findings that anti-Mart antibodies have different abilities to inhibit cell-cell adhesion, to enhance the respiratory burst of neutrophils, and to recognize different epitopes at the N-terminal region of CD11b. In conclusion, some anti-Mart alloantibodies interfere with Mac-1-dependent cellular functions of neutrophils, cause NAIN, and may be used as tools for studying Mac-1-dependent functions.
Keyword Integrin MAC-1 CD11B/CD18
Surface-adherent platelets
BETA-propeller domain
Glycoprotein IB-ALPHA
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Wed, 22 Sep 2010, 09:46:51 EST