Two-dimensional size/branch length distributions of a branched polymer

Vilaplana, Francisco and Gilbert, Robert G. (2010) Two-dimensional size/branch length distributions of a branched polymer. Macromolecules, 43 17: 7321-7329. doi:10.1021/ma101349t


Author Vilaplana, Francisco
Gilbert, Robert G.
Title Two-dimensional size/branch length distributions of a branched polymer
Journal name Macromolecules   Check publisher's open access policy
ISSN 0024-9297
1520-5835
Publication date 2010-09-14
Sub-type Article (original research)
DOI 10.1021/ma101349t
Volume 43
Issue 17
Start page 7321
End page 7329
Total pages 9
Editor Timothy P. Lodge
Place of publication Washington, DC, U.S.A.
Publisher American Chemical Society
Collection year 2011
Language eng
Subject 03 Chemical Sciences
09 Engineering
C1
Formatted abstract
The first two-dimensional structural distributions are reported for a hyperbranched polymer, the two dimensions being macromolecular size and individual branch length. The 2D distributions for native starch, a polymer with both short-and long-chain branched components (amylopectin and amylose), were obtained by size fractionation using size-exclusion chromatography combined with enzymatic debranching. These distributions show distinct macromolecular architectures: separate "mountains" corresponding to amylopectin and amylose and two "foothills" assigned to hybrid populations. The distributions reveal new mechanistic information on the underlying polymer synthesis. The branch-length distributions for amylopectin are independent of macromolecular size, whereas a size variation is observed for amylose. Biological imperatives in amylopectin biosynthesis force the same branching structure for all macromolecular sizes because of evolutionary pressure to provide the crystallinity indispensable for the plant survival. Amylose biosynthesis does not have such restrictive biological constraints, offering a range of branched structures throughout macromolecular sizes and revealing a previously unsuspected size dependence.
© 2010 American Chemical Society.
Keyword Size-exclusion chromatography
Molecular-weight distributions
Assisted carbohydrate electrophoresis
Laser-light scattering
Dimethyl-sulfoxide
Amylose content
Fine-structure
Chain-length
Bowel health
Rice starch
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
Centre for Nutrition and Food Sciences Publications
 
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Created: Sun, 19 Sep 2010, 00:03:49 EST