Nanopatch-targeted skin vaccination against West Nile virus and chikungunya virus in mice

Prow, Tarl W., Chen, Xianfeng, Prow, Natalie A., Fernando, Germain J. P., Tan, Cindy S. E., Raphael, Anthony P., Chang, David, Ruutu, Merja P., Jenkins, Derek W. K., Pyke, Alyssa, Crichton, Michael L., Raphaelli, Kristin, Goh, Lucas Y. H., Frazer, Ian H., Roberts, Michael S., Gardner, Joy, Khromykh, Alexander A., Suhrbier, Andreas, Hall, Roy A. and Kendall, Mark A. F. (2010) Nanopatch-targeted skin vaccination against West Nile virus and chikungunya virus in mice. Small, 6 16: 1776-1784. doi:10.1002/smll.201000331


Author Prow, Tarl W.
Chen, Xianfeng
Prow, Natalie A.
Fernando, Germain J. P.
Tan, Cindy S. E.
Raphael, Anthony P.
Chang, David
Ruutu, Merja P.
Jenkins, Derek W. K.
Pyke, Alyssa
Crichton, Michael L.
Raphaelli, Kristin
Goh, Lucas Y. H.
Frazer, Ian H.
Roberts, Michael S.
Gardner, Joy
Khromykh, Alexander A.
Suhrbier, Andreas
Hall, Roy A.
Kendall, Mark A. F.
Title Nanopatch-targeted skin vaccination against West Nile virus and chikungunya virus in mice
Journal name Small   Check publisher's open access policy
ISSN 1613-6810
1613-6829
Publication date 2010-08
Sub-type Article (original research)
DOI 10.1002/smll.201000331
Volume 6
Issue 16
Start page 1776
End page 1784
Total pages 9
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag
Collection year 2011
Language eng
Formatted abstract
The 'Nanopatch' (NP) comprises arrays of densely packed projections with a defined geometry and distribution designed to physically target vaccines directly to thousands of epidermal and dermal antigen presenting cells (APCs). These miniaturized arrays are two orders of magnitude smaller than standard needles - which deliver most vaccines-and are also much smaller than current microneedle arrays. The NP is dry-coated with antigen, adjuvant, and/or DNA payloads. After the NP was pressed onto mouse skin, a protein payload co-localized with 91.4 ±4.1 APC mm-2 (or 2925 in total) representing 52% of the delivery sites within the NP contact area, agreeing well with a probability-based model used to guide the device design; it then substantially increases as the antigen diffuses in the skin to many more cells. APC colocalizing with protein payloads rapidly disappear from the application area, suggesting APC migration. The NP also delivers DNA payloads leading to cutaneous expression of encoded proteins within 24 h. The efficiency of NP immunization is demonstrated using an inactivated whole chikungunya virus vaccine and a DNA-delivered attenuated West Nile virus vaccine. The NP thus offers a needle-free, versatile, highly effective vaccine delivery system that is potentially inexpensive and simple to use.
© 2010 Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim.
Keyword Immunology
Medicine
Microstructures
Transcutaneous
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 27 JUL 2010

 
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Created: Sun, 19 Sep 2010, 00:01:41 EST