Functional genomic and serological analysis of the protective immune response resulting from vaccination of macaques with an NS1-truncated influenza virus

Baskin, C. R., Bielefeldt-Ohmann, H., García-Sastre, A., Tumpey, T. M., Van Hoeven, N., Carter, V. S., Thomas, M. J., Proll, S., Solorzano, A., Billharz, R., Fornek, J.L., Thomas, S., Chen, C.-H., Clark, E.A., Murali-Krishna, K. and Katze, M.G. (2007) Functional genomic and serological analysis of the protective immune response resulting from vaccination of macaques with an NS1-truncated influenza virus. Journal of Virology, 81 21: 11817-11827. doi:10.1128/JVI.00590-07


Author Baskin, C. R.
Bielefeldt-Ohmann, H.
García-Sastre, A.
Tumpey, T. M.
Van Hoeven, N.
Carter, V. S.
Thomas, M. J.
Proll, S.
Solorzano, A.
Billharz, R.
Fornek, J.L.
Thomas, S.
Chen, C.-H.
Clark, E.A.
Murali-Krishna, K.
Katze, M.G.
Title Functional genomic and serological analysis of the protective immune response resulting from vaccination of macaques with an NS1-truncated influenza virus
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2007-11-01
Sub-type Article (original research)
DOI 10.1128/JVI.00590-07
Open Access Status DOI
Volume 81
Issue 21
Start page 11817
End page 11827
Total pages 11
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract We are still inadequately prepared for an influenza pandemic due to the lack of a vaccine effective for subtypes to which the majority of the human population has no prior immunity and which could be produced rapidly in sufficient quantities. There is therefore an urgent need to investigate novel vaccination approaches. Using a combination of genomic and traditional tools, this study compares the protective efficacy in macaques of an intrarespiratory live influenza virus vaccine produced by truncating NS1 in the human influenza A/Texas/36/91 (H1N1) virus with that of a conventional vaccine based on formalin-killed whole virus. After homologous challenge, animals in the live-vaccine group had greatly reduced viral replication and pathology in lungs and reduced upper respiratory inflammation. They also had lesser induction of innate immune pathways in lungs and of interferon-sensitive genes in bronchial epithelium. This postchallenge response contrasted with that shortly after vaccination, when more expression of interferon-sensitive genes was observed in bronchial cells from the live-vaccine group. This suggested induction of a strong innate immune response shortly after vaccination with the NS1-truncated virus, followed by greater maturity of the postchallenge immune response, as demonstrated with robust influenza virus-specific CD4+ T-cell proliferation, immunoglobulin G production, and transcriptional induction of T- and B-cell pathways in lung tissue. In conclusion, a single respiratory tract inoculation with an NS1-truncated influenza virus was effective in protecting nonhuman primates from homologous challenge. This protection was achieved in the absence of significant or long-lasting adverse effects and through induction of a robust adaptive immune response.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Erratum: Functional genomic and serological analysis of the protective immune response resulting from vaccination of macaques with an NS1-truncated influenza virus (vol 81, pg 1181, 2007) vol 81 (21): 11817.

Document type: Journal Article
Sub-type: Article (original research)
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