Lung tumor development and spontaneous regression in lambs coinfected with Jaagsiekte sheep retrovirus and ovine lentivirus

Hudachek, S. F., Kraft, S. L., Thamm, D. H., Bielefeldt-Ohmann, H., DeMartini, J. C., Miller, A. D. and Dernell, W. S. (2010) Lung tumor development and spontaneous regression in lambs coinfected with Jaagsiekte sheep retrovirus and ovine lentivirus. Veterinary Pathology, 47 1: 148-162. doi:10.1177/0300985809352787


Author Hudachek, S. F.
Kraft, S. L.
Thamm, D. H.
Bielefeldt-Ohmann, H.
DeMartini, J. C.
Miller, A. D.
Dernell, W. S.
Title Lung tumor development and spontaneous regression in lambs coinfected with Jaagsiekte sheep retrovirus and ovine lentivirus
Journal name Veterinary Pathology   Check publisher's open access policy
ISSN 0300-9858
1544-2217
Publication date 2010-01-01
Sub-type Article (original research)
DOI 10.1177/0300985809352787
Open Access Status Not Open Access
Volume 47
Issue 1
Start page 148
End page 162
Total pages 15
Place of publication Thousand Oaks, CA, United States
Publisher Sage Publications
Collection year 2011
Language eng
Formatted abstract
Ovine pulmonary adenocarcinoma (OPA) is a naturally occurring and experimentally inducible lung cancer of sheep caused by Jaagsiekte sheep retrovirus (JSRV). The first aim of this study was to monitor the development of OPA with minimally invasive, real-time observations of animals experimentally infected with JSRV as well as ovine lentivirus (maedi-visna virus). Worldwide, simultaneous infection of sheep with these 2 retroviruses is a common occurrence, naturally and experimentally; consequently, the lung tumor homogenates used as inocula contained both viruses. Following inoculation, computed tomography was used to detect tumor nodules early, before the onset of clinical signs, and to monitor tumor advancement. However, not only was OPA disease progression observed, but the apparent spontaneous regression of OPA was witnessed. In fact, regression was more common than progression following JSRV inoculation of neonatal lambs. Immune responses were detected, particularly involving CD3+ T cells and the production of antibodies against JSRV that may mediate the spontaneous regression of JSRV-induced OPA. The second aim of this study was to determine whether OPA tumors harbor genetic alterations similar to those found in human lung adenocarcinoma. No mutations were found in the tyrosine kinase domain of the epidermal growth factor receptor, KRAS codons 12 and 13, or the DNA-binding domain of p53 in tumor DNA from naturally occurring and experimentally-induced OPA cases. Overall, the genetic profile combined with the disease development data provides further important characterization of OPA and describes, for the first time, spontaneous regression of OPA tumors in experimentally infected sheep. © The American College of Veterinary Pathologists 2010.
Keyword Computed tomography
Jaagsiekte sheep retrovirus
Ovine pulmonary adenocarcinoma
Spontaneous tumor regression
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Veterinary Science Publications
 
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Created: Wed, 15 Sep 2010, 11:32:02 EST by Laura McTaggart on behalf of School of Veterinary Science