F2 excimer laser (157 nm) radiation modification and surface ablation of PHEMA hydrogels and the effects on bioactivity: Surface attachment and proliferation of human corneal epithelial cells

Zainuddin, Chirila, Traian V., Barnard, Zeke, Watson, Gregory S., Toh, Chiong, Blakey, Idriss, Whittaker, Andrew K. and Hill, David J. T. (2011). F2 excimer laser (157 nm) radiation modification and surface ablation of PHEMA hydrogels and the effects on bioactivity: Surface attachment and proliferation of human corneal epithelial cells. In: O. Guven, D.J.T. Hill and S. Tagawa, Radiation Modification Of Polymers, 11th Pacific Polymer Conference. 11th Pacific Polymer Conference, Cairns, Australia, (219-229). 6-10 December 2009. doi:10.1016/j.radphyschem.2010.07.036


Author Zainuddin
Chirila, Traian V.
Barnard, Zeke
Watson, Gregory S.
Toh, Chiong
Blakey, Idriss
Whittaker, Andrew K.
Hill, David J. T.
Title of paper F2 excimer laser (157 nm) radiation modification and surface ablation of PHEMA hydrogels and the effects on bioactivity: Surface attachment and proliferation of human corneal epithelial cells
Conference name 11th Pacific Polymer Conference
Conference location Cairns, Australia
Conference dates 6-10 December 2009
Proceedings title Radiation Modification Of Polymers, 11th Pacific Polymer Conference   Check publisher's open access policy
Journal name Radiation Physics and Chemistry   Check publisher's open access policy
Place of Publication Oxford, United Kingdom
Publisher Pergamon
Publication Year 2011
Year available 2010
Sub-type Fully published paper
DOI 10.1016/j.radphyschem.2010.07.036
ISSN 0969-806X
1879-0895
Editor O. Guven
D.J.T. Hill
S. Tagawa
Volume 80
Issue 2
Start page 219
End page 229
Total pages 11
Collection year 2011
Language eng
Abstract/Summary Physical and chemical changes at the surface of poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogels modified by ablation with an F2 excimer laser were investigated experimentally. An important observation was that only the outer exposed surface layers of the hydrogel were affected by the exposure to 157 nm radiation. The effect of the surface changes on the tendency of cells to adhere to the PHEMA was also investigated. A 0.5 cm2 area of the hydrogel surfaces was exposed to laser irradiation at 157 nm to fluences of 0.8 and 4 J cm−2. The changes in surface topography were analysed by light microscopy and atomic force microscopy, while the surface chemistry was characterized by attenuated total reflection infrared and X-ray photoelectron spectroscopies. Cell-interfacial interactions were examined based on the proliferation of human corneal limbal epithelial (HLE) cells cultured on the laser-modified hydrogels, and on the unexposed hydrogels and tissue culture plastic for comparison. It was observed that the surface topography of laser-exposed hydrogels showed rippled patterns with a surface roughness increasing at the higher exposure dose. The changes in surface chemistry were affected not only by an indirect effect of hydrogen and hydroxyl radicals, formed by water photolysis, on the PHEMA, but also by the direct action of laser radiation on PHEMA if the surface layers of the gel become depleted of water. The laser treatment led to a change in the surface characteristics, with a lower concentration of ester side-chains and the formation of new oxygenated species at the surface. The surface also became more hydrophobic. Most importantly, the surface chemistry and the newly created surface topographical features were able to improve the attachment, spreading and growth of HLE cells. Copyright © 2010 Elsevier Ltd All rights reserved.
Subjects 030306 Synthesis of Materials
030603 Colloid and Surface Chemistry
030304 Physical Chemistry of Materials
Keyword Excimer laser
PHEMA hydrogel
Role of water
Surface chemistry
Surface topography
Cell attachment
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 3 August 2010.

 
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Created: Mon, 13 Sep 2010, 11:28:24 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging