High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: Intra-serovar divergence, interserovar convergence, and evidence of attenuation in Leptospira reference collections

Tulsiani, SM, Craig, SB, Graham, GC, Cobbold, RC, Dohnt, MF, Burns, MA, Jansen, CC, Leung, LKP, Field, HE and Smythe, LD (2010) High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: Intra-serovar divergence, interserovar convergence, and evidence of attenuation in Leptospira reference collections. Annals of Tropical Medicine and Parasitology, 104 5: 427-437. doi:10.1179/136485910X12786389891047


Author Tulsiani, SM
Craig, SB
Graham, GC
Cobbold, RC
Dohnt, MF
Burns, MA
Jansen, CC
Leung, LKP
Field, HE
Smythe, LD
Title High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: Intra-serovar divergence, interserovar convergence, and evidence of attenuation in Leptospira reference collections
Formatted title
High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: Intra-serovar divergence, interserovar convergence, and evidence of attenuation in Leptospira reference collections
Journal name Annals of Tropical Medicine and Parasitology   Check publisher's open access policy
ISSN 0003-4983
1364-8594
Publication date 2010-07
Sub-type Article (original research)
DOI 10.1179/136485910X12786389891047
Volume 104
Issue 5
Start page 427
End page 437
Total pages 11
Place of publication London, U.K.
Publisher Maney Publishing
Collection year 2011
Language eng
Formatted abstract
High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) is a novel technology that has emerged as a possible method to characterise leptospires to serovar level. RAPD-HRM has recently been used to measure intra-serovar convergence between strains of the same serovar as well as inter-serovar divergence between strains of different serovars. The results indicate that intra-serovar heterogeneity and inter-serovar homogeneity may limit the application of RAPD-HRM in routine diagnostics. They also indicate that genetic attenuation of aged, high-passage-number isolates could undermine the use of RAPD-HRM or any other molecular technology. Such genetic attenuation may account for a general decrease seen in titres of rabbit hyperimmune antibodies over time. Before RAPD-HRM can be further advanced as a routine diagnostic tool, strains more representative of the wild-type serovars of a given region need to be identified. Further, RAPD-HRM analysis of reference strains indicates that the routine renewal of reference collections, with new isolates, may be needed to maintain the genetic integrity of the collections.
© 2010 Maney Publishing.
Keyword Family leptospiraceae
SP NOV.
Serovars
Relatedness
Serogroups
Evolution
Virulence
Bacteria
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
 
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Created: Sun, 12 Sep 2010, 00:07:50 EST