Molecular analysis of the simultaneous production of two SHV-type extended-spectrum beta-lactamases in a clinical isolate of Enterobacter cloacae by using single-nucleotide polymorphism genotyping

Szabo, Dóra, Melan, Melissa A., Hujer, Andrea M., Bonomo, Robert A., Hujer, Kristine M., Bethel, Christopher R., Kristof, Katalin and Paterson, David L. (2005) Molecular analysis of the simultaneous production of two SHV-type extended-spectrum beta-lactamases in a clinical isolate of Enterobacter cloacae by using single-nucleotide polymorphism genotyping. Antimicrobial Agents and Chemotherapy, 49 11: 4716-4720. doi:10.1128/AAC.49.11.4716-4720.2005

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Author Szabo, Dóra
Melan, Melissa A.
Hujer, Andrea M.
Bonomo, Robert A.
Hujer, Kristine M.
Bethel, Christopher R.
Kristof, Katalin
Paterson, David L.
Title Molecular analysis of the simultaneous production of two SHV-type extended-spectrum beta-lactamases in a clinical isolate of Enterobacter cloacae by using single-nucleotide polymorphism genotyping
Journal name Antimicrobial Agents and Chemotherapy   Check publisher's open access policy
ISSN 0066-4804
Publication date 2005-11
Sub-type Article (original research)
DOI 10.1128/AAC.49.11.4716-4720.2005
Open Access Status File (Publisher version)
Volume 49
Issue 11
Start page 4716
End page 4720
Total pages 5
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Subject 1103 Clinical Sciences
1117 Public Health and Health Services
Formatted abstract
Bacteria that simultaneously produce multiple extended-spectrum beta-lactamases are frequently isolated. We report an Enterobacter cloacae isolate, ES24, producing four different beta-lactamases (AmpC type beta-lactamase, TEM-1, SHV-7, and a novel extended-spectrum beta-lactamase, SHV-30). Direct sequencing of P blaSHV gene products gave a "double peak" at position 703, suggesting the presence of more than one allele. Using fluorescence resonance energy transfer real-time PCR to detect single-nucleotide polymorphisms, we were able to distinguish two different blaSHV genes in a single isolate. This may prove to be a useful technique in surveys of beta-lactamase production in contemporary clinical isolates. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Keyword Strand Conformational Polymorphism
Klebsiella-pneumoniae Strains
Escherichia-coli
Gene
Hospitals
Diversity
ESBLs
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Tue, 07 Sep 2010, 17:12:44 EST