Rapid method of extraction and analysis of extended-spectrum beta-lactamases from clinical strains of Klebsiella pneumoniae

Paterson, D.L., Rice, L.B. and Bonomo, R.A. (2001) Rapid method of extraction and analysis of extended-spectrum beta-lactamases from clinical strains of Klebsiella pneumoniae. Clinical Microbiology and Infection, 7 12: 709-711. doi:10.1046/j.1469-0691.2001.00348.x


Author Paterson, D.L.
Rice, L.B.
Bonomo, R.A.
Title Rapid method of extraction and analysis of extended-spectrum beta-lactamases from clinical strains of Klebsiella pneumoniae
Journal name Clinical Microbiology and Infection   Check publisher's open access policy
ISSN 1469-0691
1198-743X
Publication date 2001-12
Sub-type Article (original research)
DOI 10.1046/j.1469-0691.2001.00348.x
Volume 7
Issue 12
Start page 709
End page 711
Total pages 3
Place of publication Oxford, England
Publisher Blackwell Science
Language eng
Subject 1103 Clinical Sciences
Abstract The extraction of periplasmic β-lactamases from Gram-negative bacilli is a necessary preliminary step to analytical isoelectric focusing. Previously described methods are time-consuming and require large amounts of broth. We describe a lysozyme-based method which needs just 5 mL broth and requires less than 24 h to perform. The method was reproducible in extracting β-lactamases from reference strains containing known β-lactamases. We applied the method to a collection of more than 70 extended-spectrum β-lactamase-producing isolates from a multinational study of bacteremic isolates of Klebsiella pneumoniae. Further studies are being undertaken to assess the method's applicability to other bacterial species.
Keyword Escherichia-coli
Resistance
Ceftazidime
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Tue, 07 Sep 2010, 17:08:22 EST