Expression of genes coding for histone variants and histone-associated proteins in pluripotent stem cells and mouse preimplantation embryos

Kafer, GR, Lehnert, SA, Pantaleon, M, Kaye, PL and Moser, RJ (2010) Expression of genes coding for histone variants and histone-associated proteins in pluripotent stem cells and mouse preimplantation embryos. Gene Expression Patterns, 10 6: 299-305. doi:10.1016/j.gep.2010.06.003


Author Kafer, GR
Lehnert, SA
Pantaleon, M
Kaye, PL
Moser, RJ
Title Expression of genes coding for histone variants and histone-associated proteins in pluripotent stem cells and mouse preimplantation embryos
Journal name Gene Expression Patterns   Check publisher's open access policy
ISSN 1872-7298
1567-133X
Publication date 2010-09-01
Sub-type Article (original research)
DOI 10.1016/j.gep.2010.06.003
Volume 10
Issue 6
Start page 299
End page 305
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2011
Language eng
Formatted abstract
The histone code is an epigenetic regulatory system thought to play a crucial role in cellular events such as development, differentiation and in the maintenance of pluripotency. In order to gain an insight into the role variant histones may play during mammalian development; we studied gene expression of histone variants and remodelling enzymes in mouse embryonic stem (ES) cells and during mouse preimplantation development. Using quantitative reverse-transcription PCR (qRT-PCR) we document the gene expression pattern of 12 histone variants and 2 of their associated remodelling enzymes in undifferentiated ES cells and during preimplantation embryo development. All histone variants were detected in undifferentiated ES cells, with H2AZ showing the highest expression levels of all the histone variants tested. The results also show that H2A variant levels tend to increase later in embryo development whilst H3 variant levels are elevated in early preimplantation stages. In addition, the expression of SWI/SNF, a remodeler protein involved in specifically remodelling H2A-H2B dimers, mirrors the expression of H2B and H2A variants, and the H3-H4 specific chaperone CAF-1 expression mirrors H3 variant expression. These results provide a foundation for further studies on the functions of histone variants during development, differentiation and in pluripotency.
Copyright 2010 Elsevier B.V. All rights reserved.
Keyword Mouse
Preimplantation embryo
Embryonic stem cells
Histone
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biomedical Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 29 Aug 2010, 10:06:26 EST