Amnion cell biosynthesis of interleukin-8: Regulation by inflammatory cytokines

Trautman, M.S., Dudley, D.J., Edwin, S.S., Collmer, D. and Mitchell, M.D. (1992) Amnion cell biosynthesis of interleukin-8: Regulation by inflammatory cytokines. Journal of Cellular Physiology, 153 1: 38-43. doi:10.1002/jcp.1041530107

Author Trautman, M.S.
Dudley, D.J.
Edwin, S.S.
Collmer, D.
Mitchell, M.D.
Title Amnion cell biosynthesis of interleukin-8: Regulation by inflammatory cytokines
Journal name Journal of Cellular Physiology   Check publisher's open access policy
ISSN 1097-4652
Publication date 1992-10
Sub-type Article (original research)
DOI 10.1002/jcp.1041530107
Volume 153
Issue 1
Start page 38
End page 43
Total pages 6
Place of publication New York, N.Y. U.S.A.
Publisher Wiley
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Abstract The cellular constituents of the placenta are important participants in the recruitment and trafficking of inflammatory cells within the placenta. In infection-induced labor, gestational tissues synthesize and release a variety of inflammatory cytokines whose effects include increased prostaglandin biosynthesis and the initiation of uterine contractions. Interleukin-8 (IL-8), a potent neutrophil chemoattractant, has been recently described as being elevated in the amniotic fluid of mothers with chorioamnionitis. We investigated the biosynthesis of IL-8 by human amnion cells and its regulation by other inflammatory cytokines. Cultured amnion cells obtained from normal term placentae were found to produce IL-8 in response to pathophysiologic concentrations of interleukin 1β (IL-1β) and tumor necrosis factor alpha (TNF-α). Treatment of amnion cells stimulated by IL-1β with cycloheximide resulted in increased IL-8 production, while incubation of IL-1β treated amnion cells with actinomycin D resulted in a concentration-dependent decrease in detectable amounts of IL-8. Northern blot analysis of cultured amnion cells stimulated with IL-1β demonstrated a rapid increase in IL-8 mRNA which peaked at 2-4 hr. These in vitro results suggest inflammation of gestational tissues in vivo may result in locally produced IL-8 and, in association with other inflammatory mediators, may be important in the pathophysiology of infection-induced labor.
Keyword Neutrophil Chemotactic Factor
Activating Peptide-1 Interleukin-8
IL-8 Gene-Expression
Preterm Labor
Prostaglandin Biosynthesis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Created: Thu, 26 Aug 2010, 13:19:07 EST