Does leptin exhibit cytokine-like properties in tissues of pregnancy?

Soh, E. B. E., Mitchell, M. D. and Keelan, J. A. (2000). Does leptin exhibit cytokine-like properties in tissues of pregnancy?. In: American Journal of Reproductive Immunology:. 46th Annual Meeting of the Society-for-Gynecologic-Investigation, Atlanta, Georgia, U.S.A., (292-298). 10-13 March, 1999. doi:10.1111/j.8755-8920.2000.430508.x


Author Soh, E. B. E.
Mitchell, M. D.
Keelan, J. A.
Title of paper Does leptin exhibit cytokine-like properties in tissues of pregnancy?
Conference name 46th Annual Meeting of the Society-for-Gynecologic-Investigation
Conference location Atlanta, Georgia, U.S.A.
Conference dates 10-13 March, 1999
Proceedings title American Journal of Reproductive Immunology:   Check publisher's open access policy
Journal name American Journal of Reproductive Immunology   Check publisher's open access policy
Place of Publication Copenhagen, Denmark
Publisher Munksgaard
Publication Year 2000
Sub-type Fully published paper
DOI 10.1111/j.8755-8920.2000.430508.x
ISSN 1046-7408
0271-7352
1600-0897
Volume 43
Issue 5
Start page 292
End page 298
Total pages 6
Language eng
Formatted Abstract/Summary
PROBLEM:
To determine whether leptin exhibits cytokine-like properties in gestational tissues in light of its homologies with the class I family of cytokines.

METHOD OF STUDY:
WISH and JEG3 cells, and amnion and choriodecidua explants, were treated inflammatory modulators (interleukin-1β [IL-1β], tumor necrosis factor-α [TNF-α] and bacterial lipopolysaccharide [LPS]) and leptin production was measured by immunoassay. Other agents known to regulate adipocyte leptin production were also tested for comparative purposes. In addition, WISH cells, JAR cells and placental explants were treated with leptin to assess its effects on production of IL-8, IL-6 and prostaglandin E2 (PGE2).

RESULTS:
Leptin production by all cells and tissues studied was unaffected by treatment with IL-1β (2.5 ng/mL), TNF-α (25 ng/mL) and LPS (2.5 μg/mL). Dexamethasone stimulated leptin production over two-fold by WISH and JEG3 cells, whereas insulin also stimulated a two-fold increase in leptin production in JEG3 cells. IL-6 production by JAR cells and placental explants was stimulated (two- to three-fold) by leptin (300 ng/mL). PGE2 production was unaffected.

CONCLUSIONS:
Leptin derived from gestational tissues is unlikely to play a role in inflammatory reactions within the placenta, but may regulate placental cytokine production. The physiological significance of amnion-derived leptin remains to be established.
Subjects 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
1115 Pharmacology and Pharmaceutical Sciences
Keyword cytokines
interleukin-6
leptin
placenta
Obese Gene-Product
Cord-Blood Leptin
Serum Leptin
Growth
Humans
Weight
Glucocorticoids
Expression
Secretion
Anorexia
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 13:17:47 EST