Labour-associated changes in the regulation of production of immunomodulators in human amnion by glucocorticoids, bacterial lipopolysaccharide and pro-inflammatory cytokines

Simpson, K.L., Keelan, J.A. and Mitchell, M.D. (1999) Labour-associated changes in the regulation of production of immunomodulators in human amnion by glucocorticoids, bacterial lipopolysaccharide and pro-inflammatory cytokines. Journal of Reproduction and Fertility, 116 2: 321-327. doi:10.1530/jrf.0.1160321


Author Simpson, K.L.
Keelan, J.A.
Mitchell, M.D.
Title Labour-associated changes in the regulation of production of immunomodulators in human amnion by glucocorticoids, bacterial lipopolysaccharide and pro-inflammatory cytokines
Journal name Journal of Reproduction and Fertility   Check publisher's open access policy
ISSN 0022-4251
Publication date 1999-07
Sub-type Article (original research)
DOI 10.1530/jrf.0.1160321
Volume 116
Issue 2
Start page 321
End page 327
Total pages 7
Place of publication Oxford, England
Publisher Blackwell Scientific Publications
Language eng
Subject 1103 Clinical Sciences
1115 Pharmacology and Pharmaceutical Sciences
Formatted abstract
Parturition is associated with changes in the production of inflammatory mediators by gestational tissues. An explant system was established to study the change in response of human amnion to various regulating factors during labour. Disks of tissue (6 mm) were excised from amnion membranes obtained either at term by Caesarian section before labour (n = 5-6) or after spontaneous vaginal delivery (n = 3-7). After 24 h equilibration in media, the tissues were treated with interleukin 1β (10 ng ml-1), tumour necrosis factor α (100 ng ml-1), lipopolysaccharide (5 μg ml-1) and dexamethasone (1 μmol l-1) or an appropriate vehicle control for 24 h (n = 3 wells per treatment). Media were harvested and interleukin 10, interleukin 6 and prostaglandin E2 concentrations were determined by immunoassay. In tissues taken both before and after the onset of labour, basal interleukin 10 production by amnion explants was near to the limit of detection. Basal production rates of PGE2 by amnion explants were significantly higher (P < 0.0012; Mann-Whitney U test) in tissues taken during labour than in tissues taken before the onset of labour, while interleukin 6 production was not significantly altered by labour. Production rates of interleukin 6 and prostaglandin E2 were significantly increased by interleukin 1β, tumour necrosis factor α and lipopolysaccharide in explants from tissues taken during and before labour, while the responsiveness of interleukin 10 production to these treatments was inconsistent. Dexamethasone had no effect on interleukin 6 production by amnion explants, but significantly inhibited prostaglandin E2 production, although this inhibition was approximately 30% lower in tissues obtained after the onset of labour. These results support the presence of inflammatory positive feedback circles, coincident with a deficiency of an anti-inflammatory factor within gestational tissue, which may be involved in the progression or maintenance of labour.
Keyword Tumor-necrosis-factor
Human Gestational Tissues
Human Fetal Membranes
Growth-factor-beta
Preterm Labor
Human Placenta
Prostaglandin Production
Fluid Interleukin - 6
Human Trophoblast
Human Parturition
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 13:17:43 EST