Stimulants of prostaglandin biosynthesis in human fetal membranes, uterine decidua vera and placenta

Saeed, S.A. and Mitchell, M.D. (1982) Stimulants of prostaglandin biosynthesis in human fetal membranes, uterine decidua vera and placenta. Prostaglandins, 24 4: 475-484. doi:10.1016/0090-6980(82)90005-3

Author Saeed, S.A.
Mitchell, M.D.
Title Stimulants of prostaglandin biosynthesis in human fetal membranes, uterine decidua vera and placenta
Journal name Prostaglandins   Check publisher's open access policy
ISSN 0090-6980
Publication date 1982-10
Sub-type Article (original research)
DOI 10.1016/0090-6980(82)90005-3
Volume 24
Issue 4
Start page 475
End page 484
Total pages 10
Place of publication Stoneham, Ma., U.S.A.
Publisher Butterworths
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Formatted abstract
Modulation of prostaglandin (PG) biosynthesis by cytosolic fractions derived from homogenates of human amnion, chorion laeve, decidua vera and placenta was examined. PGF2(α) and 6-oxo-PGF1(α) synthesis by bovine seminal vesicle (BSV) PG synthase was stimulated by the cytosolic fractions of each tissue in a dose-dependent manner. The cytosols from decidua vera and placenta were the most effective in stimulating synthesis and also stimulated PGE2 biosynthesis. Reduced glutathione (GSH) acted to increase the biosynthesis of PGE2 at the expense of other PGs both in the presence and absence of various cytosols. These data are indicative that the mode of action of cytosolic fractions on the stimulation of PG biosynthesis is unlike that of GSH. Indomethacin and aspirin, inhibitors of fatty acid cyclooxygenase activity, strongly inhibited the cytosol-induced stimulation of BSV PG synthase. The cytosolic factors that stimulated PG biosynthesis exhibited differential behavior towards boiling and dialysis. The stimulatory effect of all cytosolic fractions was sensitive to boiling except in the case of chorion laeve effects toward 6-oxo-PGF1(α) production. In dialysis studies we found that the cytosolic components that stimulated the production of PGF2(α) were not removed by dialysis except in the case of cytosol of placenta whereas the stimulatory effects of various cytosols toward the biosynthesis of PGE2 and 6-oxo-PGF1(α) were removed by dialysis. These results are indicative of the presence of endogenous factors in human intrauterine tissues that preferentially stimulate the biosynthesis of PGF2(α) and 6-oxo-PGF1(α) and are further suggestive that PG biosynthesis in intrauterine tissues is, at least in part, regulated by cytosolic factors.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 26 Aug 2010, 13:13:14 EST