Formation of arachidonate lipoxygenase metabolites by human fetal membranes, uterine decidua vera and placenta

Saeed, S. A. and Mitchell, M. D. (1982) Formation of arachidonate lipoxygenase metabolites by human fetal membranes, uterine decidua vera and placenta. Prostaglandins Leukotrienes and Medicine, 8 6: 635-640.

Author Saeed, S. A.
Mitchell, M. D.
Title Formation of arachidonate lipoxygenase metabolites by human fetal membranes, uterine decidua vera and placenta
Journal name Prostaglandins Leukotrienes and Medicine   Check publisher's open access policy
ISSN 0262-1746
Publication date 1982
Sub-type Article (original research)
Volume 8
Issue 6
Start page 635
End page 640
Total pages 6
Place of publication Edinburgh ; New York
Publisher Churchill Livingstone
Language eng
Subject 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Formatted abstract
Homogenates (1000 x g supernatant fraction) of human amnion, chorion laeve, decidua vera and placenta were incubated with [1-14C]arachidonic acid in the presence or absence of indomethacin, an inhibitor of cyclooxygenase activity and nordihydroguairetic acid (NDGA), an inhibitor of lipoxygenase activity. After incubation, the products were extracted with ethyl acetate and identified by thin layer chromatography and high performance liquid chromatography. The major lipoxygenase products formed by human amnion, decidua vera and placenta were 12-L-hydroxy-5,8,10,14-eicosatretetraenoic acid (12-HETE) and smaller amounts of 5-HETE. Chorion laeve produced only a trace amount of 12-HETE. The formation of these products was abolished by NDGA and slightly stimulated by indomethacin. These findings demonstrate the existence of lipoxygenase activity in human intra-uterine tissues and suggest that products of lipoxygenase activity in these tissues may regulate the recruitment of leukocytes and/or macrophages during pregnancy and parturition.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 13:13:02 EST