Characterization of the amnion-derived AV3 cell line for use as a model for investigation of prostaglandin-cytokine interactions in human amnion

Potter, S., Hansen, W. R., Keelan, J. A. and Mitchell, M. D. (1999) Characterization of the amnion-derived AV3 cell line for use as a model for investigation of prostaglandin-cytokine interactions in human amnion. Prostaglandins Leukotrienes and Essential Fatty Acids, 61 6: 373-379. doi:10.1054/plef.1999.0114


Author Potter, S.
Hansen, W. R.
Keelan, J. A.
Mitchell, M. D.
Title Characterization of the amnion-derived AV3 cell line for use as a model for investigation of prostaglandin-cytokine interactions in human amnion
Journal name Prostaglandins Leukotrienes and Essential Fatty Acids   Check publisher's open access policy
ISSN 0952-3278
1532-2823
Publication date 1999-12
Sub-type Article (original research)
DOI 10.1054/plef.1999.0114
Volume 61
Issue 6
Start page 373
End page 379
Total pages 7
Place of publication Edinburgh ; New York
Publisher Churchill Livingstone
Language eng
Subject 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Formatted abstract
Increased production of prostaglandins and cytokines by amnion, particularly prostaglandin (PG) E2, interleukin (IL)-6 and IL-8, is thought to be an important event in infection-associated preterm labour. We characterized the amnion-derived AV3 cell line to determine its appropriateness as a model for investigation of the regulation of amnion cytokine and PG production. Amnion-derived AV3 cells were treated with tumour necrosis factor-α (TNF-α, interleukin-1β (IL-1β), epidermal growth factor (EGF) and phorbol 12-myristate 13-acetate (PMA) and IL-6, IL-8 and prostaglandin production was determined by immunoassay. Production of IL-6 and IL-8 rose dramatically with all treatments. PGE2, but not PGF(2α) or 6-keto- PGF(1α), biosynthesis was also increased in a concentration- dependent manner with all treatments. A rapid increase in PGHS-2 (but not PGHS-1) mRNA expression was observed in response to TNF-α and IL-1β. We conclude that the AV3 cell line inflammatory response profile is similar to those observed in primary amnion and other amnion-derived cell lines, and is an appropriate model for human amnion. (C) 1999 Harcourt Publishers Ltd.
Keyword Tumor-necrosis-factor
Preterm Labor
Messenger-rna
Factor-alpha
Wish Cells
Gestational Tissues
Interleukin (IL)-6
IL-8 Production
Phorbol Esters
Growth-factors
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 13:10:15 EST