Interleukin-1 beta (IL-1 beta) modulates prostaglandin production and the natural IL-1 receptor antagonist inhibits ovulation in the optimally stimulated rat ovarian perfusion model

Peterson, C.M., Hales, H.A., Hatasaka, H.H., Mitchell, M.D., Rittenhouse, L. and Jones, K.P. (1993) Interleukin-1 beta (IL-1 beta) modulates prostaglandin production and the natural IL-1 receptor antagonist inhibits ovulation in the optimally stimulated rat ovarian perfusion model. Endocrinology, 133 5: 2301-2306. doi:10.1210/en.133.5.2301


Author Peterson, C.M.
Hales, H.A.
Hatasaka, H.H.
Mitchell, M.D.
Rittenhouse, L.
Jones, K.P.
Title Interleukin-1 beta (IL-1 beta) modulates prostaglandin production and the natural IL-1 receptor antagonist inhibits ovulation in the optimally stimulated rat ovarian perfusion model
Journal name Endocrinology   Check publisher's open access policy
ISSN 0013-7227
Publication date 1993-11
Sub-type Article (original research)
DOI 10.1210/en.133.5.2301
Volume 133
Issue 5
Start page 2301
End page 2306
Total pages 6
Place of publication Bethesda, MA, U.S.A.
Publisher Endocrine Society
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Formatted abstract
The rat ovarian perfusion model with bursa removed and intact was used to further characterize the effects of interleukin-1β (IL-1β) and the natural IL-1 receptor antagonist (IRAP) on ovulation, steroidogenesis, and prostaglandin production. Twenty-six- to 27-day-old female Sprague-Dawley rats were injected sc with 25 IU PMSG, and 48 h later, the right ovary was dissected (with bursa removed and intact for various experiments) and placed in the perfusion chamber. Ovaries were exposed to various doses of IL-1β alone, IL-1β with LH, and IL-1β with LH and isobutylmethylxanthine (IBMX). The natural IL-1 receptor antagonist was also added to the chambers with LH and IBMX. IL-1β at 0.8 (n = 3) and 8.0 (n = 4) nM did not induce LH- independent ovulation in PMSG-stimulated ovaries with bursa removed. In bursa-intact perfusions (n = 3), one ovulation was produced in each compared to control ovaries with bursa intact (n = 3) given an ovulatory trigger of LH alone [2.3 ± 0.6 (±SD) ovulations; P < 0.02]. IL-1β enhanced, in a dose- and gonadotropin-dependent fashion, the production of prostaglandin E2 (PGE2) in PMSG-stimulated ovaries with bursa removed given an ovulatory trigger of LH and IBMX compared to that in controls. PGF(2α) and 6-keto- PGF(1α) were also modulated by IL-1β. Estradiol and progesterone production were not affected. The natural IRAP inhibited ovulation (7.8 ± 3.9 ovulations vs. 12.4 ± 1.5; P < 0.04) in PMSG-stimulated ovaries given LH and IBMX as the ovulatory trigger compared to that in controls. This inhibition of ovulation was not associated with reduced steroid or PG levels. IL-1β appears to play a potentially significant role in the process of ovulation. The functional importance of the bursa in this model is highlighted in this study. IL-1β modulates PG, but not steroid, production. IRAP inhibited ovulation without significantly affecting PG or steroid production.
Keyword Porcine Granulosa-Cells
Hormone-Induced DifferentIation
Cytokine-Mediated Regulation
Human Synovial-Cells
Endothelial-cells
Progesterone Secretion
Procoagulant Activity
Vascular Endothelium
Expression
Gonadotropin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 13:09:52 EST