A sensitive radioimmunoassay for 6-keto-prostaglandin F1α: preliminary observations on circulating concentrations

Mitchell, M.D. (1978) A sensitive radioimmunoassay for 6-keto-prostaglandin F1α: preliminary observations on circulating concentrations. Prostaglandins and Medicine, 1 1: 13-21. doi:10.1016/0161-4630(78)90072-1


Author Mitchell, M.D.
Title A sensitive radioimmunoassay for 6-keto-prostaglandin F1α: preliminary observations on circulating concentrations
Formatted title
A sensitive radioimmunoassay for 6-keto-prostaglandin F: preliminary observations on circulating concentrations
Journal name Prostaglandins and Medicine   Check publisher's open access policy
ISSN 0161-4630
Publication date 1978-07
Sub-type Article (original research)
DOI 10.1016/0161-4630(78)90072-1
Volume 1
Issue 1
Start page 13
End page 21
Total pages 9
Place of publication New York, N.Y. U.S.A.
Publisher Churchill Livingstone
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Formatted abstract
A radioimmunoassay for 6-keto-prostaglandin F (1α) (6-keto-PGF (1α)) is reported. The mean least detectable mass of 6-keto-PGF (1α) is 1.45 pg and the mean mass of added 6-keto-PGF (1α) required to displace zero-point binding by 50% is 94.2 pg. Cross-reactivities of the antiserum with all the prostaglandins and related compounds tested are <0.1% except for PGE2 and PGF(2α) which are respectively 0.16% and 0.80%. The assay is both accurate and reproducible and measured levels are in good agreement following extraction of 0.25-4.0 ml of plasma. Mean circulating concentrations of 6-keto-PGF (1α) in five non-pregnant humans, rhesus monkeys and sheep were 115, 65 and 25 pg/ml respectively.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 13:00:52 EST