Regulation of prostaglandin biosynthesis in dispersed choriodecidual cells in culture

Marvin, K. W. and Mitchell, M. D. (2001) Regulation of prostaglandin biosynthesis in dispersed choriodecidual cells in culture. Prostaglandins Leukotrienes and Essential Fatty Acids, 65 5-6: 247-251. doi:10.1054/plef.2001.0321


Author Marvin, K. W.
Mitchell, M. D.
Title Regulation of prostaglandin biosynthesis in dispersed choriodecidual cells in culture
Journal name Prostaglandins Leukotrienes and Essential Fatty Acids   Check publisher's open access policy
ISSN 0952-3278
1532-2823
Publication date 2001-11-01
Sub-type Article (original research)
DOI 10.1054/plef.2001.0321
Volume 65
Issue 5-6
Start page 247
End page 251
Total pages 5
Place of publication Edinburgh ; New York
Publisher Churchill Livingstone
Language eng
Subject 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Formatted abstract
We have evaluated the prostaglandin (PG) production and PG biosynthetic gene expression in a choriodecidual dispersed cell culture system. Cells dispersed from human choriodecidual membranes by dispase and trypsin digestion were evaluated after 1, 3, 5 and 7 days of culture for basal and tumour necrosis factor α (F-α) stimulated PGE2 production. The highest rates of production (P < 0.05) were obtained with cells treated after 3 days of culture, (3.7 ±1 ) × 102 pg PGE2 per 16 h per μg total cellular protein (mean ± SEM), which was 3.9 times basal rate after 3 days culture. In choriodecidual cells treated after 3 days in culture, expression of prostaglandin endoperoxide H synthase-2 (PGHS-2) mRNA was similarly responsive to TNF-α (3.9 times basal within 3 h of 30 ng/ml TNF-α) while there was little effect on PGHS-1 or cytosolic phospholipase A2 expression. Hence, the dispersed choriodecidual cell culture system described retains TNF-α responsive PG biosynthetic capacity which is at least in part upregulated via increased expression of PGHS-2 mRNA. © 2001 Harcourt Publishers Ltd.
Keyword Necrosis-factor-alpha
Synthase Pghs Activity
Preterm Labor
Human Amnion
Messenger-rna
Gestational Tissues
Human Chorion
Human Decidua
H Synthase-2
Cyclooxygenase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 23:00:03 EST