Expression of angiogenic and neurotrophic factors in the human amnion and choriodecidua

Marvin, K. W., Keelan, J. A., Eykholt, R. L., Sato, T. A. and Mitchell, M. D. (2002) Expression of angiogenic and neurotrophic factors in the human amnion and choriodecidua. American Journal of Obstetrics and Gynecology, 187 3: 728-734. doi:10.1067/mob.2002.126286

Author Marvin, K. W.
Keelan, J. A.
Eykholt, R. L.
Sato, T. A.
Mitchell, M. D.
Title Expression of angiogenic and neurotrophic factors in the human amnion and choriodecidua
Journal name American Journal of Obstetrics and Gynecology   Check publisher's open access policy
ISSN 0002-9378
Publication date 2002-09
Sub-type Article (original research)
DOI 10.1067/mob.2002.126286
Volume 187
Issue 3
Start page 728
End page 734
Total pages 7
Place of publication St. Louis, Mo. U.S.A.
Publisher Mosby
Language eng
Subject 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Formatted abstract
Our objective was to identify the novel or differential expression of growth or development associated genes in the human gestational membranes that might play roles in pregnancy or in term or preterm parturition.


Complementary DNA arrays were probed with [α33p]dCTP-labeled-complementary DNA that was prepared from the RNA of reflected amnion and choriodecidua that represent term not-in-labor, term spontaneous labor, and preterm labor with and without chorioamnionitis (n = 4 per group). Differential expression (term not-in-labor vs term spontaneous labor or preterm labor with chorioamnionitis vs preterm labor without chorioamnionitis) was evaluated by Wilcoxon tests.

All 16 amnion samples expressed angiogenic factors (endothelin-2 and -3, vascular endothelial growth factor, and vascular endothelial growth factor-B) and neurotrophic factors (ephrin-A2, ephrin receptors-A2, -B1, -B3, -B4, and -B5, neuropilin-2, p75/nerve growth factor receptor and semaphorin-F). In both amnion and choriodecidua, the expression of vascular endothelial growth factor and the angiopoietin receptor, Tie-2, were greater with term spontaneous labor than with term not-in-labor (P < .05); increased VEGF receptor-2 (flk-1) expression was observed in term spontaneous labor choriodecidua (P < .05) but not amnion. Ephrin-A1 expression increased with term spontaneous labor in both tissues (P < .05). Semaphorin-F expression decreased with preterm labor with chorioamnionitis in choriodecidua (P < .05), although the trend was not significant in amnion (P = .1).


Neurotrophic and angiogenic factor genes are expressed in amnion and choriodecidual membranes. Several of the genes exhibit differential expression with labor at term or in association with infection preterm, which suggests roles in or associated with these processes.
Keyword Amnion
Expression array
Gestational tissue
Endothelial Growth-factor
Adhesion Molecule-1 Icam-1
Preterm Labor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 51 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 26 Aug 2010, 12:59:46 EST